Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages

• Published in: Bulletin of experimental biology and medicine Vol. 166; no. 3; pp. 386 - 389
• Main Authors: Pirutin, S. K., Efremova, M. V., Yusipovich, A. I., Turovetskii, V. B., Maksimov, G. V., Druzhko, A. B., Mazhuga, A. G.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-01-2019; Springer Nature B.V
• Subjects: Antigens; Biomedical and Life Sciences; Biomedicine; Biotechnologies; Cell Biology; Cytoplasm; Cytotoxicity; Gold; Internal Medicine; Laboratory Medicine; Macrophages; Magnetite; Nanoparticles; Nanotechnology; Oncology; Pathology; Prostate; Therapeutic applications; cell membranes; macrophages; magnetite-gold nanoparticles; prostate-specific membrane antigen
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-019-04356-9

We demonstrated the possibility of penetration of magnetite-gold nanoparticles conjugated with prostate-specific membrane antigen into mouse macrophages. It was found that after 3-h incubation with nanoparticles in a concentration of 15 mg/liter at 37oC, they were seen in only 13% macrophages. In about 90% cells, the nanoparticles were detected within the cytoplasm. Under these conditions, membrane damage was revealed in 25% cells. These results should be taken into account in further development and application of nanomaterials for diagnostic and therapeutic purposes in oncology.