The effect of teprenone on the intestinal morphology and microbial community of Chinese sea bass (Lateolabrax maculatus) under intermittent hypoxic stress

• Published in: Fish physiology and biochemistry Vol. 46; no. 5; pp. 1873 - 1882
• Main Authors: Dong, Hong-biao, Sun, Yong-xu, Duan, Ya-fei, Li, Hua, Li, Yong, Liu, Qing-song, Wang, Wen-hao, Zhang, Jia-song
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-10-2020; Springer Nature B.V
• Subjects: Abundance; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Anti-Ulcer Agents - pharmacology; Bacilli; Bacteria - classification; Bacteria - drug effects; Bacteroidetes; Bass; Biomedical and Life Sciences; Cyanobacteria; Diterpenes - pharmacology; Fish; Freshwater & Marine Ecology; Gastrointestinal Microbiome - drug effects; Histology; Hypoxia; Intestine; Intestines; Intestines - drug effects; Intestines - pathology; Juveniles; Lateolabrax maculatus; Life Sciences; Marine fishes; Microbiomes; Microorganisms; Morphology; Mucosa; Next-generation sequencing; Proteobacteria; Relative abundance; Sea bass; Survival; Technology assessment; Zoology; Geranylgeranylacetone; Hypoxic stress; Teprenone; Microbial community; Lateolabrax maculatus
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-020-00838-0

Hypoxia stress may affect the fish intestine and thereby threaten the growth and survival of the fish. Teprenone is a clinically effective agent in protecting gastrointestinal mucosa. This study aims to assess the effect of teprenone in the intestine of Chinese sea bass
Lateolabrax maculatus
under intermittent hypoxic stress.
L. maculatus
juveniles were either raised under intermittent hypoxic condition or normal condition (NC). Part of the hypoxic-intervened fish were treated with teprenone at different concentrations (HTs), and the rest were regarded as hypoxic control (HC). Histological analysis was performed on the epithelial tissue of the fish intestine. High-throughput sequencing technology was used to analyze the diversity and composition of the microbial community in
L. maculatus
intestine. Reduced villi length and goblet cell, exfoliated enterocyte, and improper arrangement of villi were observed in HC compared with NC and HTs.
Proteobacteria
,
Firmicutes
, and
Bacteroidetes
represented the most abundant phyla in each sample. Significantly higher microbial diversity was detected in HC compared with NC (
P
 < 0.05). At the phylum level, HC presented significantly decreased relative abundance of
Proteobacteria
, and significantly increased relative abundance of
Bacteroidetes
,
Chloroflex
, and
Cyanobacteria
compared with NC (
P
 < 0.05). At the class level, HC showed significantly reduced relative abundance of
Alphaproteobacteria
and
Bacilli
, and significantly increased relative abundance of
Clostridia
,
Gammaproteobacteria
, and
Bacteroides
(
P
 < 0.05). Teprenone protects the intestine from epithelial damages and maintains the microbial harmony in
L. maculatus
under intermittent hypoxic stress.