Bradyrhizobium uaiense sp. nov., a new highly efficient cowpea symbiont

• Published in: Archives of microbiology Vol. 202; no. 5; pp. 1135 - 1141
• Main Authors: Cabral Michel, Daniele, Azarias Guimarães, Amanda, Martins da Costa, Elaine, Soares de Carvalho, Teotonio, Balsanelli, Eduardo, Willems, Anne, Maltempi de Souza, Emanuel, de Souza Moreira, Fatima Maria
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-07-2020; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Bradyrhizobium; Cell Biology; Cowpeas; Deoxyribonucleic acid; DNA; DnaK protein; Ecology; Genes; Life Sciences; Microbial Ecology; Microbiology; New species; Nucleotides; Original Paper; Phylogeny; RecA protein; rRNA 16S; Plant growth-promoting bacteria; Phylogeny; Biological nitrogen fixation; Taxonomy; Bradyrhizobium
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-01827-w

This study describes two
Bradyrhizobium
strains
,
UFLA03-164
T
and UFLA03-153, which share more than 99% sequence similarity of the 16S rRNA with the type strains of 15 species in this genus. The concatenation of three housekeeping genes (
recA
,
gyrB
, and
dnaK
) indicated that both strains formed a single clade separate from known
Bradyrhizobium
species.
B. viridifuturi
, represented by SEMIA 690
T
, is the closest neighboring species (96.2%). Low (< 92%) average nucleotide identity (ANI) was observed between strain UFLA03-164
T
and any of the closest species on the phylogenetic trees based on concatenated housekeeping genes. The DNA G+C content of UFLA03-164
T
is 63.25%. Phenotypic characteristics were determined for both UFLA strains. Based on the data, the two strains represent a new species for which the name
Bradyrhizobium uaiense
is proposed, with UFLA03-164
T
(= LMG 31509
T
) as type strain.