Dietary pectin caused great changes in bile acid profiles of Pelteobagrus fulvidraco

• Published in: Fish physiology and biochemistry Vol. 47; no. 6; pp. 2015 - 2025
• Main Authors: Cao, Xiamin, Ren, Shengjie, Cai, Chunfang, Ni, Qin, Li, Xinyue, Meng, Yunhe, Meng, Zijing, Shi, Ye, Chen, Huangen, Jiang, Rong, Wu, Ping, Ye, Yuantu
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Bile; Bile Acids and Salts - analysis; Biomedical and Life Sciences; Catfish; Catfishes; Dextrin; Diet; Diet - veterinary; Dietary fiber; Dietary fibres; Fish; Fish diets; Fish meal; Fishmeal; Freshwater & Marine Ecology; Freshwater fishes; Glycine; Glycine (amino acid); Hindgut; Histology; Homeostasis; Life Sciences; Liver; Morphology; Pectin; Pectins - administration & dosage; Pelteobagrus fulvidraco; Reabsorption; Serum; Tandem Mass Spectrometry; Taurine; Zoology; Pectin; Yellow catfish; Dietary fiber; Bile acid profile
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-021-01028-2

To reveal the impact of dietary fiber (DF) on the bile acid (BA) profiles of fish, yellow catfish (
Pelteobagrus fulvidraco
) were fed a diet containing 300 g kg
−1
dextrin (CON diet, control) or pectin (a type of soluble DF, PEC diet) for 7 days, and then the BA profiles were analyzed by UHPLC-MS/MS. A total of 26 individuals of BAs were detected in the fish body, with 8, 10, 14, and 22 individuals of BAs detected in the liver, serum, bile, and hindgut digesta, respectively. The conjugated BAs (CBAs) of fish were dominated by taurine CBAs (TCBAs). The concentrations of free BAs (FBAs) and the value of FBAs/CBAs in the bile of fish fed the PEC diet were nearly 5 and 7 times higher, respectively than those in fish fed the CON diet. The value of glycine CBAs/TCBAs in the liver, serum and bile of fish fed the PEC diet was significantly lower, and in the hindgut digesta was higher than that of fish fed the CON diet (
P
 < 0.05). These results suggested that dietary pectin greatly changed the BA profiles of
Pelteobagrus fulvidraco
, attributed to inhibition of reabsorption of BAs. Therefore, attention should be paid to the impact on BA homeostasis when replacing fishmeal with DF-rich plant ingredients in the fish diet.