Evaluation of carcinogenic activities and sperm abnormalities of Gram-negative bacterial metabolites isolated from cancer patients after subcutaneous injection in albino rats

• Published in: Antonie van Leeuwenhoek Vol. 114; no. 3; pp. 287 - 302
• Main Authors: El-Gendy, Mervat Morsy Abbas Ahmed, Abdel-Wahhab, Khaled G., Hassan, Nabila S., El-Bondkly, Esraa Ahmed, Farghaly, Ayman A., Ali, Hanan F., Ali, Sanaa A., El-Bondkly, Ahmed M. A.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-03-2021; Springer Nature B.V
• Subjects: Abnormalities; Alanine; Alanine transaminase; Alkaline phosphatase; Antigens; Aspartate aminotransferase; Bacteria; Biomedical and Life Sciences; Cancer; Carcinoembryonic antigen; Carcinogens; Creatinine; Cytotoxicity; Erythrocytes; Evaluation; Gram-negative bacteria; Hepatotoxicity; Inflammation; Injection; Interleukins; L-Lactate dehydrogenase; Lactate dehydrogenase; Lactic acid; Life Sciences; Medical Microbiology; Metabolites; Microbiology; Micronuclei; Microorganisms; Original Paper; Phospholipids; Plant Sciences; Secondary metabolites; Serum levels; Soil Science & Conservation; Sperm; Statistical analysis; Toxicity; Tumor markers; Tumor necrosis factor-α; Tumorigenesis; Urea; Multidrug resistance bacteria; Nephrotoxicity; Hepatotoxicity; Carcinogenesis; Sperm anomalies
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01522-w

Microbial pathogens drive tumorigenesis in 20% of cancer cases, so the present study is aimed to evaluate the carcinogenic activities, sperm abnormalities and other dangerous effects of the subcutaneous injection of extracts obtained from various clinical Gram-negative bacteria derived from cancer patients using albino rats. We isolated, identified and extracted of their secondary metabolites of carbapenem resistant Gram-negative bacteria derived from cancer patients. Various methods have been used to determine hepatotoxicity, nephrotoxicity, tumorigenesis, inflammatory and sperm abnormalities in the albino rats injected with extracts. In comparison with the normal animals group, all extracts induced hepatotoxicity which was evidenced by the significant elevation in the activity of the serum alanine aminotransferase, aspartate aminotransferase, gamma-glutamyltransferase and alkaline phosphatase; also, nephrotoxicity that was indicated through the marked increase in the serum urea and creatinine levels; tumorigenesis was achieved from the sharp elevation in serum levels of alpha fetoprotein, carcinoembryonic antigen and lactate dehydrogenase values as tumor markers; as well as severe inflammatory characteristics were monitored from the marked raise of tumor necrosis factor alpha and interleukin-1beta. Furthermore, the proportion of micronuclei in polychromatic erythrocytes and sperm abnormalities were statistically significant in all groups compared to control group. Various kinds of head abnormalities and coiled tail were noted. Histopathological examination of hepatic tissue came in line with the biochemical and cytological findings. It could conclude that the extracts of
Serratia
sp. Esraa 1,
Stenotrophomonas
sp. Esraa 2,
Acinetobacter
sp. Esraa 3,
Escherichia
sp. Esraa 4 and
Pseudomonas
sp. Esraa 5 were able to initiate cytotoxicity and tumorigenesis in rats.