Recent advances of sensing strategies for the detection of β-glucuronidase activity

• Published in: Analytical and bioanalytical chemistry Vol. 414; no. 9; pp. 2935 - 2951
• Main Authors: Li, Tong, Li, Guoliang, Su, Zhuoqun, Liu, Jianghua, Wang, Panxue
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2022; Springer Nature B.V
• Subjects: Acids; Analytical Chemistry; Animals; Bacteria; Biochemistry; Biomarkers; Body fluids; Cancer; Carbon; Catalysis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Colorimetry; E coli; Electrochemistry; Environmental health; Enzymes; Feces; Fluorescence; Food Science; Glucuronidase; Hydrogels; Hydrolase; Laboratory Medicine; Mammals; Metabolism; Microbiota; Monitoring/Environmental Analysis; Nonsteroidal anti-inflammatory drugs; Physiology; Quantum dots; Review; Sensors; Trends; Tumors; Water monitoring; Water Quality; Glucuronidase activity; Biomarker; Tumor diseases; Microbiological quality of water; β-Glucuronidase activity; Escherichia coli
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-022-03921-y

β
-Glucuronidase (
β
-GLU), a kind of hydrolase, is widely distributed in mammalian tissues, body fluids, and microbiota. Abnormal changes of
β
-GLU activity are often correlated with the occurrence of diseases and deterioration of water quality. Therefore, detection of
β
-GLU activity is of great significance in biomedicine and environmental health such as cancer diagnosis and water monitoring. However, the conventional
β
-GLU activity assay suffers from the limitations of low sensitivity, poor accuracy, and complex procedure. With the development of analytical chemistry, many advances have been made in the detection of
β
-GLU activity in recent years. The sensors for
β
-GLU activity detection which have the advantages of rapid and reliable detection have been attracting increased attentions. In this paper, the principles, performances, and limitations of these
β
-GLU sensors, including colorimetric sensing, fluorescent sensing, electrochemical sensing for the determination of
β
-GLU activity, have been summarized and discussed. Moreover, the challenges and research trends of
β
-GLU activity assay are proposed.
Graphical abstract