The effect of bee bread (Perga) with chemotherapy on MDA-MB-231 cells

• Published in: Molecular biology reports Vol. 48; no. 3; pp. 2299 - 2306
• Main Authors: Caner, Armagan, Onal, Muge Gulcihan, Silici, Sibel
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-03-2021; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Antioxidants; Apoptosis; Bcl-2 protein; Bee bread; Bees; BH3 Interacting Domain Death Agonist Protein - genetics; BH3 Interacting Domain Death Agonist Protein - metabolism; BID gene; Biomedical and Life Sciences; Breast cancer; Cancer; Cell Line, Tumor; Cell migration; Cell Movement - drug effects; Cell proliferation; Cell Proliferation - drug effects; Chemotherapy; Cisplatin; Cisplatin - pharmacology; Doxorubicin; Doxorubicin - pharmacology; Gene Expression Regulation, Neoplastic - drug effects; Histology; Humans; Life Sciences; Morphology; Original Article; Propolis - pharmacology; Royal jelly; Toxicity; Wound healing; Bee Bread; Breast Cancer; Doxorubicin; Cisplatin
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06259-3

Bee bread (BB) is a bee product like propolis and honey. It is the main food for larvae and bees producing royal jelly in the hive. It also known as Perga. As with other bee products, it is increasingly popular due to its antioxidant properties. The aim of this study was to examine the effects of BB on MDA-MB-231 breast cancer cells and the effects on these cells when administered together with Doxorubicin (DOX) and Cisplatin (CDDP), used in cancer treatment. The proliferation of the cells was determined by applying 5 mg/mL BB together with different concentrations of DOX and CDDP. In addition to these studies, the effect of DOX+BB and CDDP+BB combinations on the migration of MDA-MB-231 cells was determined by the wound healing method. The expression levels of Bid and Bcl-2 were determined by RtqPCR. According to these studies, as expected, BB did not show a significant toxic effect on MDA-MB-231 cells at different concentrations. BB significantly suppressed the effect of DOX and CDDP on the proliferation of MDA-MB-231 cells. BB with DOX and CDDP suppressed the proapoptotic Bid gene while overexpressing the anti-apoptotic Bcl-2 gene, separately. Interestingly, BB blocked the migration of MDA-MB-231 cells by 50% even after 72 h. As a result, BB significantly reduced the toxicity of DOX and CDDP on MDA-MB-231 cells. The most interesting result of the study is that BB prevented the migration of cancer cells.