Characterization of extracellular secondary metabolites in Oudemansiella canarii BRM-044600 displaying antifungal activity against the phytopathogen Sclerotinia sclerotiorum

• Published in: World journal of microbiology & biotechnology Vol. 37; no. 12; p. 203
• Main Authors: de Oliveira Vieira, Vandinelma, Conceição, Aparecido Almeida, de Oliveira Gorgulho Silva, Caio, Romero-Pelaez, Ruben Dario, Junior, Murillo Lobo, Abdelnur, Patrícia Verardi, de Almeida, João Ricardo Moreira, Almeida, Euziclei Gonzaga, de Siqueira, Félix Gonçalves
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2021; Springer Nature B.V
• Subjects: Agaricales - metabolism; Antifungal activity; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Applied Microbiology; Ascomycota - drug effects; Basidiomycota; Bioactive compounds; Biochemistry; Biological control; Biological Control Agents - pharmacology; Biomedical and Life Sciences; Biotechnology; Economic importance; Environmental Engineering/Biotechnology; Fatty Acids, Unsaturated - metabolism; Filtrate; Fungi; Fungicides; Fungicides, Industrial - pharmacology; Life Sciences; Liquid culture; Metabolites; Microbial Sensitivity Tests; Microbiology; Mold; Mycelia; Mycelium - drug effects; Mycelium - growth & development; Original Paper; Plant Diseases - microbiology; Sclerotinia sclerotiorum; Secondary Metabolism; Secondary metabolites; Stereoisomerism; Stereoisomers; Strobilurin; Strobilurins - metabolism; White mold; Basidiomycetes; Plant pathogen; Strobilurins; Biocontrol; Fungal secondary metabolites
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-021-03172-6

White mold disease, caused by the phytopathogen
Sclerotinia sclerotiorum
, provokes severe productivity losses in several economically important crops. Biocontrol agents, especially antagonist filamentous fungi, are environmentally friendly alternatives to the chemical fungicides used in white mold management. The objective of this study was to screen for basidiomycete fungi capable of inhibiting
S. sclerotiorum
and investigate their bioactive metabolites responsible for antifungal activities. Two out of 17 tested basidiomycete isolates inhibited the mycelial growth of
S. sclerotiorum
in pair culture experiments on agar plates, namely
Oudemansiella canarii
BRM-044600 and
Laetisaria arvalis
ATCC52088.
O. canarii
BRM-044600 liquid culture filtrate exhibited the greatest antifungal activity and was selected for further investigation. UHPLC-MS analysis suggests that six putative strobilurins, including strobilurin A and/or stereoisomers of this compound (m/z 259.1299, [M + H]
+
) and three putative strobilurins with m/z 257.1184 ([M + H]
+
) are likely responsible for the antifungal activity observed in the culture filtrate. For the first time, this work demonstrated the potential of
O. canarii
for white mold biocontrol and strobilurin production.
Graphic abstract