Prophylactic effect of myricetin and apigenin against lipopolysaccharide-induced acute liver injury

• Published in: Molecular biology reports Vol. 48; no. 9; pp. 6363 - 6373
• Main Authors: Berköz, Mehmet, Ünal, Seda, Karayakar, Fahri, Yunusoğlu, Oruç, Özkan-Yılmaz, Ferbal, Özlüer-Hunt, Arzu, Aslan, Ali
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-09-2021; Springer Nature B.V
• Subjects: Administration, Oral; Animal Anatomy; Animal Biochemistry; Animals; Apigenin - administration & dosage; Bilirubin; Biomedical and Life Sciences; Catalase; Catalase - blood; Chemical and Drug Induced Liver Injury - blood; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - prevention & control; Cyclooxygenase-2; Cytokines - blood; Disease Models, Animal; Flavonoids; Flavonoids - administration & dosage; Glutathione - blood; Hepatitis, Animal - prevention & control; Histology; IL-1β; Inflammation; Interleukin 6; JNK protein; Life Sciences; Lipopolysaccharides; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - adverse effects; Liver; Liver Function Tests; Male; Mice; Mice, Inbred BALB C; Morphology; mRNA; NF-κB protein; Nitric-oxide synthase; Oral administration; Original Article; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Pre-Exposure Prophylaxis - methods; Prostaglandin E2; Protective Agents - administration & dosage; Sepsis; Serum Albumin - analysis; Superoxide dismutase; Superoxide Dismutase - blood; Treatment Outcome; Tumor necrosis factor-α; Lipopolysaccharide; Oxidative stress; Inflammation; Acute liver injury; Apigenin; Myricetin
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-021-06637-x

Background
Liver has an important role in the initiation and progression of multiple organ failure that occurs in sepsis. Many natural active substances can be used to reduce the liver injury caused by sepsis. For this aim, the effects of myricetin and apigenin on mice model of acute liver injury was evaluated in this study.
Methods and results
Thirty-six mice were randomly divided into six groups as; control, lipopolysaccharide (LPS) (5 mg/kg), LPS + myricetin (100 mg/kg), LPS + myricetin (200 mg/kg), LPS + apigenin (100 mg/kg), and LPS + apigenin (200 mg/kg) groups. Myricetin and apigenin were administered orally for 7 days, and LPS was administered intraperitoneally only on the 7th day of the study. 24 h after LPS application, all animals were sacrificed and serum biochemical parameters, histopathology and oxidative stress and inflammation markers of liver tissue were examined. Myricetin and apigenin pre-treatments increased serum albumin and total protein levels, liver GSH level and catalase and SOD activities and decreased serum ALT, AST, ALP, γ-GT, CRP, total and direct bilirubin levels, liver MPO activity, MDA, NOx, PGE
2
, TNF-α, IL-1β, and IL-6 levels,
iNOS
and
COX-2
mRNA levels, phosphorylation of NF-κB p65, IκB, and IKK proteins but not p38, ERK, and JNK proteins in LPS-treated mice. Myricetin and apigenin administration also regained the hepatic architecture disrupted during LPS application.
Conclusion
Myricetin and apigenin pre-treatments led to reduction of liver injury indices and oxidative stress and inflammatory events and these flavonoids has probably hepatoprotective effects in acute liver injury.