Chemical Hypoxic Preconditioning Improves Survival and Proliferation of Mesenchymal Stem Cells

• Published in: Applied biochemistry and biotechnology Vol. 196; no. 7; pp. 3719 - 3730
• Main Authors: Haneef, Kanwal, Salim, Asmat, Hashim, Zehra, Ilyas, Amber, Syed, Basir, Ahmed, Aftab, Zarina, Shamshad
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-07-2024; Springer Nature B.V
• Subjects: Actin; Animals; Biochemistry; Biotechnology; Cell Hypoxia; Cell Proliferation; Cell Survival; Chemistry; Chemistry and Materials Science; Dehydrogenase; Dehydrogenases; Glucose 1-dehydrogenase; Glucosephosphate dehydrogenase; Glutamate dehydrogenase; Humans; Hypoxia; In vivo methods and tests; Malate dehydrogenase; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Molecular modelling; Original Article; Peroxiredoxin; Phosphopyruvate hydratase; Preconditioning; Protein disulfide-isomerase; Proteins; Proteomics; Regeneration (physiology); Stem cell transplantation; Stem cells; Superoxide dismutase; Survival; Tissue engineering; Hypoxia; Cell survival; Proliferation; Preconditioning; Mesenchymal Stem Cells
• ISSN: 0273-2289; 1559-0291; 1559-0291
• DOI: 10.1007/s12010-023-04743-z

Increasing evidence has demonstrated that mesenchymal stem cells (MSCs) have been linked to tissue regeneration both in vitro and in vivo. However, poor engraftment and low survival rate of transplanted MSCs are still a major concern. It has been found that the proliferation, survival, and migration of MSCs are all increased by hypoxic preconditioning. However, the molecular mechanism through which hypoxic preconditioning enhances these beneficial properties of MSCs remains to be fully investigated. Therefore, the present study is aimed to investigate the mechanism by which hypoxic preconditioning enhances the survival of MSCs. We used proteomic analysis to explore the molecules that may contribute to the survival and proliferation of hypoxic preconditioned (HP) MSCs. The analysis revealed a higher expression of prelamin A/C (Lmna), glutamate dehydrogenase 1(Glud1), Actin, cytoplasmic 1(Actb), Alpha-enolase (Eno1), Glucose-6-phosphate 1-dehydrogenase (G6pd), Protein disulfide-isomerase A3 (Pdia3), Malate dehydrogenase (Mdh1), Peroxiredoxin-6 (Prdx6), Superoxide dismutase (Sod1), and Annexin A2 (Anxa2) in HP-MSCs. These proteins are possibly involved in cellular survival and proliferation through various cellular pathways. This research could aid in understanding the processes involved in hypoxic preconditioning of MSCs and designing of cell-based therapeutic strategies for tissue regeneration.