Prenatal Determination of Fetal RhD Type

• Published in: The New England journal of medicine Vol. 330; no. 11; pp. 795 - 796
• Main Authors: Simsek, Suat, Bleeker, Paulien M.M, von dem Borne, Albert E.G. Kr, Bennett, Phillip, Warwick, Ruth, Cartron, Jean-Pierre
• Format: Journal Article
• Language: English
• Published: United States Massachusetts Medical Society 17-03-1994
• Subjects: Base Sequence; DNA Primers; Female; Fetal Diseases - blood; Fetal Diseases - diagnosis; Humans; Molecular Sequence Data; Polymerase Chain Reaction; Prenatal Diagnosis; Rh-Hr Blood-Group System - genetics
• ISSN: 0028-4793; 1533-4406
• DOI: 10.1056/NEJM199403173301122

To the Editor:
In the August 26 issue of the Journal, Bennett and colleagues describe the application of the polymerase chain reaction to determine the RhD genotype of fetuses by using DNA derived from chorionic villi and amniocytes
1
. The first set of oligonucleotide primers used (A1 and A2) yields a 136-base pair (bp) product common to the RhCcEe and RhD genes, whereas the second set (A3, a consensus primer, and A4, a primer specific for the 3' noncoding sequence of the RhD gene) yields a 186-bp product only in RhD-positive subjects.
Using the same primers on DNA derived from . . .