Factors affecting recovery of latent herpes simplex virus from human trigeminal ganglia

Factors affecting recovery of latent herpes simplex virus from human trigeminal ganglia

The rate of recovery of herpes simplex virus (HSV) from human trigeminal ganglia explant monolayers is affected by two factors: (1) time elapsed from the death of an individual to the establishment of in vitro culture of ganglia and (2) surface area onto which ganglia are explanted. Spontaneous reac...

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Bibliographic Details
Published in:Canadian journal of microbiology Vol. 28; no. 1; p. 123
Main Authors: Lewis, M E, Warren, K G, Jeffrey, V M, Shnitka, T K
Format: Journal Article
Language:English
Published: Canada 01-01-1982
Subjects:
Adolescent
Adult
Aged
Child
Culture Techniques
Death
DNA Restriction Enzymes
DNA, Viral
Female
Humans
Male
Middle Aged
Simplexvirus - growth & development
Simplexvirus - isolation & purification
Time Factors
Trigeminal Ganglion - microbiology
Trigeminal Nerve - microbiology
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Summary:The rate of recovery of herpes simplex virus (HSV) from human trigeminal ganglia explant monolayers is affected by two factors: (1) time elapsed from the death of an individual to the establishment of in vitro culture of ganglia and (2) surface area onto which ganglia are explanted. Spontaneous reactivation of HSV from human trigeminal ganglia can be maximized when ganglia are obtained within 12 h of death and explanted into surface area of 250 cm2. Viruses isolated by explantation of human trigeminal ganglia were found to be uniformly HSV type 1 by restriction enzyme analysis.
ISSN:0008-4166
DOI:10.1139/m82-013