Proposed method for agglutinating antibody titer analysis and its use as indicator of acquired immunity in pacu, Piaractus mesopotamicus

• Published in: Brazilian journal of biology Vol. 74; no. 1; pp. 238 - 242
• Main Authors: Biller-Takahashi, J D, Montassier, H J, Takahashi, L S, Urbinati, E C
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Internacional de Ecologia 01-02-2014
• Subjects: acquire immunology; Adaptive Immunity - immunology; Adjuvants, Immunologic - administration & dosage; Aeromonas hydrophila - immunology; Agglutination Tests - veterinary; Animals; Antibodies, Bacterial - blood; BIOLOGY; Characidae - classification; Characidae - immunology; fish; Fish Diseases - immunology; Fish Diseases - prevention & control; humoral immunity; immunology; Levamisole - administration & dosage; methodology; humoral immunity; acquire immunology; metodologia; imunologia; imunidade humoral; fish; peixe; imunidade adquirida; immunology; methodology
• ISSN: 1519-6984; 1678-4375; 1678-4375; 1519-6984
• DOI: 10.1590/1519-6984.21312

Antibody can be assessed by agglutinating antibody titer which is a quantitative measure of circulating antibodies in serum from fish previously immunized. The antibody evaluation has been performed with different fish species, and is considered a reliable method that can be applied to confirm several hypothesis regarding acquired immunity, even in conjunction with precise methods to describe immune mechanisms. In order to provide appropriate analytical methods for future studies on the specific immune system of native fish, the present study standardized on assay to measure the serum agglutinating antibody titer produced after immunization with inactivated A. hydrophila and levamisole administration in pacu. It was possible to determine the agglutinating antibodies titer in a satisfactorily way in pacu immunized with inactive A. hydrophila, and the highest titers were observed on fish fed with levamisole.