Recent advances in the use of stimulated Raman scattering in histopathology

• Published in: Analyst (London) Vol. 146; no. 3; p. 789
• Main Authors: Lee, Martin, Herrington, C Simon, Ravindra, Manasa, Sepp, Kristel, Davies, Amy, Hulme, Alison N, Brunton, Valerie G
• Format: Journal Article
• Language: English
• Published: England 07-02-2021
• Subjects: Eosine Yellowish-(YS); Hematoxylin; Nucleic Acids; Spectrum Analysis, Raman; Vibration
• ISSN: 1364-5528
• DOI: 10.1039/d0an01972k

Stimulated Raman histopathology (SRH) utilises the intrinsic vibrational properties of lipids, proteins and nucleic acids to generate contrast providing rapid image acquisition that allows visualisation of histopathological features. It is currently being trialled in the intraoperative setting, where the ability to image unprocessed samples rapidly and with high resolution offers several potential advantages over the use of conventional haematoxylin and eosin stained images. Here we review recent advances in the field including new updates in instrumentation and computer aided diagnosis. We also discuss how other non-linear modalities can be used to provide additional diagnostic contrast which together pave the way for enhanced histopathology and open up possibilities for in vivo pathology.