Effect of pre-analytical variables on glycohemoglobin measurements in routine clinical care

• Published in: Clinical biochemistry Vol. 37; no. 9; pp. 836 - 839
• Main Authors: Camargo, Joı́za L., Felisberto, Mariane, Gross, Jorge L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-09-2004
• Subjects: Analysis of Variance; Blood Chemical Analysis - methods; Blood Chemical Analysis - standards; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Glycated hemoglobin; Glycated Hemoglobin A - analysis; Glycated Hemoglobin A - standards; Humans; Labile fraction; Metabolic control; Sample storage; Specimen Handling - methods; Specimen Handling - standards; Glycated hemoglobin; Sample storage; Metabolic control; Labile fraction
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2004.03.016

Objectives: To analyze the contribution of the labile A1C fraction (L-A1C) and sample storage conditions on GHb measurements.
Design and methods: L-A1C separation (HPLC Hitachi L-9100) was evaluated before and after sample treatment by four different methods for L-A1C removal. Different sample storage conditions were analyzed.
Results: L-A1C may overestimate up to 1.64% total A1C if not properly separated. Samples were stable for up to 10, <1, and at least 100 days for whole blood stored at 4, −20, and −80 °C, respectively.
Conclusions: The presence of L-A1C may overestimate GHb results. Samples may be kept under refrigeration up to 10 days and at −80 °C if long-term storage is required.