Sequence and phylogenetic analyses of the chloroplast 16S rRNA, tufA, and rbcL genes from Bryopsis hypnoides

• Published in: Chinese journal of oceanology and limnology Vol. 29; no. 5; pp. 922 - 929
• Main Author: 吕芳 王广策
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01-09-2011; Springer Nature B.V
• Subjects: 16S; Algae; Avrainvillea; Biogeography; Biology; Bryopsidales; Bryopsis; Bryopsis hypnoides; Caulerpa sertularioides; Chlorophyta; Chloroplasts; Cladistics; Derbesia; DNA sequencing; Earth and Environmental Science; Earth Sciences; Endemism; Genes; Geographical distribution; Introns; Limnology; Marine; Nucleotide sequence; Oceanography; Phylogenetics; Phylogeny; rbcL; rRNA 16S; rRNA基因; rRNA序列; Sequencing; TufA gene; Udoteaceae; 叶绿体; 基因序列; 系统发育分析; 钙华; INW, China, People's Rep., Shandong Prov., Qingdao; phylogenetic analysis; A gene; biogeographic divergence; Bryopsidales
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-011-0084-9

Using shotgun sequencing data, the complete sequences of chloroplast 16S rRNA and tufA genes were acquired from native specimens ofBryopsis hypnoides （Qingdao, China）. There are two group I introns in the 16S rRNA gene, which is structurally similar to that of Caulerpa sertularioides （Bryopsidales, Chlorophyta）. The chloroplast-encoded tufA gene sequence is 1 230 bp long, very AT-rich （61.5%）, and is similar to previously published 16S rRNA sequences of bryopsidinean algae. Phylogenetic analyses based on chloroplast 16S rRNA and tufa gene sequence data support previous hypotheses that the Bryopsidineae, Halimedineae, and Ostreobidineae are three distinct lineages. These results also confirmed the exclusion of Avrainvillea from the family Udoteaceae. Phylogenetic analyses inferred that the genus Bryopsis as sister to Derbesia; however, this clade lacked robust nodal support. Moreover, the phylogenetic tree inferred from rbcL GenBank sequences, combined with the geographical distributions of Bryopsis species, identified a strongly supportive clade for three differently distributed Asian Bryopsis species. The preliminary results suggesting that these organisms are of distinct regional endemism.