Colloidal Fluorophore Aggregates for the Selective Detection of Albumins in Solution and on Electrophoresis Gels

• Published in: Chembiochem : a European journal of chemical biology Vol. 20; no. 3; pp. 350 - 354
• Main Authors: Park, Jihee, Kim, Youngmi
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-02-2019
• Subjects: Aggregates; aggregation; Albumins; Dismantling; Electrophoresis; Fluorescence; Fluorescent indicators; Gels; Human serum albumin; imaging agents; proteins; Reagents; self-assembly; Serum albumin; Signal transduction; Urine; self-assembly; aggregation; imaging agents; fluorescence; proteins
• ISSN: 1439-4227; 1439-7633
• DOI: 10.1002/cbic.201800608

We report a fluorescent probe that is highly sensitive and selective for serum albumins. Signal transduction results from the disassembly of fluorescence‐quenched aggregates upon binding to human serum albumin (HSA). The probe offers a rapid (≤2 s) fluorometric assay of HSA in phosphate‐buffered saline, urine, or blood serum with a high signal‐to‐noise ratio. It is also useful as a wash‐free prestaining reagent for detecting HSA on electrophoresis gels.
Switch on the light: A fluorescent probe that is highly sensitive and selective for serum albumins is reported. Disassembly of fluorescence‐quenched aggregates upon binding to human serum albumin (HSA) induces a signal. The probe offers a rapid fluorometric assay of HSA in biological fluids with a high signal‐to‐noise ratio.