Impact of herpetic stromal immune keratitis in corneal biomechanics and innervation

• Published in: Graefe's archive for clinical and experimental ophthalmology Vol. 256; no. 1; pp. 155 - 161
• Main Authors: Marcos-Fernández, Manuel Ángel, Tabernero, Sara Sánchez, Herreras, Jose María, Galarreta, David José
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 2018; Springer Nature B.V
• Subjects: Biomechanics; Confocal microscopy; Cornea; Eye; Innervation; Keratitis; Mechanical properties; Mechanical stimuli; Medicine; Medicine & Public Health; Nerves; Ophthalmology; Structure-function relationships; Corneal biomechanics; Herpetic stromal keratitis; Herpetic immune keratitis; Corneal innervation
• ISSN: 0721-832X; 1435-702X
• DOI: 10.1007/s00417-017-3826-3

Purpose
To study corneal innervation in eyes with history of herpetic keratitis and its correlation with corneal sensitivity and biomechanical properties.
Methods
A total of 56 eyes were included, of which 16 had a history of unilateral immune stromal herpetic keratitis, 16 were their contralateral eyes, and 20 were healthy controls. Structural analysis of corneal nerve plexus was performed by confocal microscopy. Biomechanical properties were measured with the Ocular Response Analyzer. Corneal sensitivity was assessed by contact (Cochet–Bonnet) and non-contact (Belmonte) esthesiometry.
Results
The eyes with a history of herpetic keratitis had reduced sensitivity for mechanical stimuli when compared to healthy eyes (1441.88 ± 83 ml/min vs. 67.9 ± 7.86 ml/min). Nerve fiber density in the corneas with a history of herpetic disease was lower (4.13 ± 2.19 U/image) than in the contralateral eyes (7.44 ± 2.9 U/image,
p
 value = 0.01) and than in healthy controls (10.35 ± 2.01,
p
 value < 0.0001). The best structural and functional correlation was established between the total length of nerves per section and mechanic threshold assessed by Belmonte esthesiometer (Coef. -0.58
p
 value < 0.0001) and between total length of nerves and corneal resistance factor (CRF) (Coef. -0.64,
p
 value < 0.0001).
Conclusions
The corneal sensitivity impairment in eyes with immune stromal herpetic keratitis can be explained by the loss of nerve fibers. Biomechanical corneal properties are affected as well. Corneal hysteresis (CH) and CRF are lower for the eyes with a history of herpetic keratitis, and also for the contralateral eye when compared to healthy controls.