AT1 receptor blockage impairs NF-κB activation mediated by thyroid hormone in cardiomyocytes

• Published in: Pflügers Archiv Vol. 470; no. 3; pp. 549 - 558
• Main Authors: Takano, Ana Paula Cremasco, Senger, Nathalia, Munhoz, Carolina Demarchi, Barreto-Chaves, Maria Luiza Morais
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2018; Springer Nature B.V
• Subjects: Angiotensin; Angiotensin II; Angiotensin II Type 1 Receptor Blockers - pharmacology; Animals; Biomedical and Life Sciences; Biomedicine; Calcium-binding protein; Calgranulin A - genetics; Calgranulin A - metabolism; Cardiomegaly - etiology; Cardiomegaly - metabolism; Cardiomyocytes; Cardiovascular system; Cell Biology; Cell culture; Cells, Cultured; Heart; Heart diseases; Human Physiology; Hyperthyroidism; Hyperthyroidism - complications; Hyperthyroidism - metabolism; Hypertrophy; Inflammation; Losartan - pharmacology; Male; Molecular Medicine; MyD88 protein; Myeloid Differentiation Factor 88 - genetics; Myeloid Differentiation Factor 88 - metabolism; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Neurosciences; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Rats; Rats, Wistar; Receptor, Angiotensin, Type 1 - metabolism; Receptors; Renin; Rodents; Serum levels; Signaling and Cell Physiology; Thyroid gland; Thyroid hormones; Thyroid Hormones - blood; Thyroid Hormones - pharmacology; Transcription activation; Cardiac hypertrophy; NF-κB; Thyroid hormone; AT1R; MyD88; S100A8
• ISSN: 0031-6768; 1432-2013
• DOI: 10.1007/s00424-017-2088-6

We have previously demonstrated that calcium-binding protein S100A8 and myeloid differentiation factor-88 (MyD88) are important mediators of nuclear transcription factor kappa-B (NF-κB) activation in cardiomyocytes and that signalling molecules are involved in the hypertrophic response that is stimulated by thyroid hormones (TH). Angiotensin II (Ang II), the main active peptide of the renin-angiotensin system (RAS), binds to type 1 Ang II receptor (AT1R) and subsequently promotes cardiac hypertrophy and the inflammatory response with NF-κB activation underlying the cardiovascular effects. Considering the amount of evidence that RAS is an important mediator of TH actions on the cardiovascular system, we aimed to investigate whether cardiac expression of NF-κB and upstream associated molecules could be altered in hyperthyroidism, as well as whether AT1R could mediate the effects of TH on cardiac tissue and in cardiomyocytes in culture. Wistar rats were subjected to hyperthyroidism with or without the AT1R blocker losartan. The TH serum levels, haemodynamic parameters and cardiac mass were assessed to confirm the hyperthyroid status. The S100A8, MyD88 and nuclear NF-κB expression levels were increased in the hearts of the hyperthyroid rats, and the losartan treatment attenuated these TH effects. In addition, the cultured cardiomyocytes that had been stimulated with losartan exhibited blunted S100A8 upregulation and NF-κB activation compared with the TH-treated cells. Together, our results suggest that AT1R participates in TH-induced cardiac hypertrophy partly by mediating S100A8, MyD88 and NF-κB activation via TH. These findings indicate the important crosstalk between TH and RAS, highlighting the participation of AT1R in the triggered mechanisms of TH that contribute to the cardiac hypertrophy response.