The inhibitory effect of dinitrosyl iron complexes (NO donors) on myeloperoxidase activity

• Published in: Doklady. Biochemistry and biophysics Vol. 477; no. 1; pp. 389 - 393
• Main Authors: Akentieva, N. P., Sanina, N. A., Gizatullin, A. R., Shmatko, N. Y., Goryachev, N. S., Shkondina, N. I., Prikhodchenko, T. R., Aldoshin, S. M.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2017; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Adhesion - drug effects; Cells, Cultured; Enzyme Activation - drug effects; Inhibitor drugs; Iron; Iron - pharmacology; Life Sciences; Molecular Biology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - enzymology; Nitrogen Oxides - pharmacology; Peroxidase; Peroxidase - antagonists & inhibitors; Peroxidase - metabolism; Rats; Sulfur
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672917060126

The effect of synthetic analogues of dinitrosyl mononuclear iron complexes (DNICs) with functional sulfur-containing ligands (NO donors) on the activity of myeloperoxidase (MPO) was studied, and their efficiency was evaluated. It was shown that the enzyme MPO is the molecular target of DNICs. It was found that six DNICs inhibited the activity of MPO and one compound potentiated it. The evaluation of their efficiency showed that two DNICs effectively inhibited the activity of MPO by 50% at IC
50
= 2 × 10
–4
M and IC
50
= 5 × 10
–7
M.