Serum Prolidase and IGF-1 as Non-invasive Markers of Hepatic Fibrosis During Four Different Periods After Bile-duct Ligation in Rats

• Published in: Digestive diseases and sciences Vol. 53; no. 7; pp. 1938 - 1945
• Main Authors: Tarçın, Orhan, Gedik, Nursal, Karakoyun, Berna, Tahan, Veysel, Sood, Gagan, Çelikel, Çiğdem, Tözün, Nurdan
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-07-2008; Springer
• Subjects: Animals; Bile Ducts; Biochemistry; Biological and medical sciences; Biomarkers - blood; Dipeptidases - blood; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Hepatology; Insulin-Like Growth Factor I - metabolism; Ligation; Liver Cirrhosis - blood; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Medicine; Medicine & Public Health; Metabolic diseases; Obesity; Oncology; Original Paper; Other diseases. Semiology; Rats; Rats, Wistar; Regression Analysis; Statistics, Nonparametric; Transplant Surgery; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Hepatic fibrosis; Serum marker; IGF-1; Collagen; Bile-duct ligation; Prolidase; Biliary tract; Obesity; Nutrition; Rat; Rodentia; Nutrition disorder; Biological marker; Hepatic disease; Metabolic diseases; Insulin like growth factor 1; Vertebrata; Mammalia; Serum marker . Bile-duct ligation; Animal; Gastroenterology; Digestive diseases; Serum; Nutritional status
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-007-0073-1

Aim
Our aim was to study the correlation of serum prolidase and insulin like growth factor-1 to liver collagen and assess their utility as markers of fibrosis during four different periods of hepatic injury and fibrosis after bile-duct ligation in rats.
Methods
Forty-eight Wistar albino rats were included in the study and divided into six groups. Seven rats served as the control group (Control), while seven rats had a sham operation (Sham group). Thirty-four rats underwent bile-duct ligation. Bile-duct ligated (BDL) animals were sacrificed at the end of the first week (Group 1;
n
 = 8), second week (Group 2;
n
 = 8), third week (Group 3;
n
 = 9), or fourth week (Group 4;
n
 = 9) after BDL. Liver collagen, liver prolidase, and serum prolidase and IGF-I, were determined.
Results
There was a positive correlation between liver collagen and serum prolidase (r
s
: 0.843,
P 
< 0.001) levels and a negative correlation among liver collagen and serum IGF-1 levels (r
s
: −0.667,
P 
< 0.001). The peak levels of liver collagen and serum prolidase were reached in the third week while the lowest levels of IGF-1 were found at the end of the third week.
Conclusion
Serum prolidase and IGF-1 either independently or in combination correlate with liver collagen content in hepatic fibrosis.