Effect of ultraviolet-B radiation in laboratory on morphological and ultrastructural characteristics and physiological parameters of selected cultivar of Oryza sativa L

• Published in: Protoplasma Vol. 250; no. 6; pp. 1303 - 1313
• Main Authors: de Almeida, Sérgio L., Schmidt, Éder C., Pereira, Debora Tomazi, Kreusch, Marianne, de L. Felix, Marthiellen R., Osorio, Luz K. P., de Paula Martins, Roberta, Latini, Alexandra, Ramlov, Fernanda, Chow, Fungyi, Maraschin, Marcelo, Rodrigues, Ana C., Bouzon, Zenilda L.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-12-2013; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cell Biology; Life Sciences; Original Article; Oryza - physiology; Oryza - radiation effects; Oryza - ultrastructure; Photosynthesis - radiation effects; Pigments, Biological - metabolism; Plant Epidermis - cytology; Plant Epidermis - radiation effects; Plant Epidermis - ultrastructure; Plant Leaves - anatomy & histology; Plant Leaves - radiation effects; Plant Proteins - metabolism; Plant Sciences; Polyphenols - metabolism; Solubility; Ultraviolet Rays; Zoology; Ice cultivar; Leaf morphology; Photosynthetic pigments; Phenolic compounds; Ultraviolet B radiation
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-013-0512-0

Ultraviolet-B radiation (UVBR) affects plants in many important ways, including reduction of growth rate and primary productivity, and changes in ultrastructures. Rice (
Oryza sativa
) is one of the most cultivated cereals in the world, along with corn and wheat, representing over 50 % of agricultural production. In this study, we examined
O
.
sativa
plants exposed to ambient outdoor radiation and laboratory-controlled photosynthetically active radiation (PAR) and PAR + UVBR conditions for 2 h/day during 30 days of cultivation. The samples were studied for morphological and ultrastructural characteristics, and physiological parameters. PAR + UVBR caused changes in the ultrastructure of leaf of
O
.
sativa
and leaf morphology (leaf index, leaf area and specific leaf area, trichomes, and papillae), plant biomass (dry and fresh weight), photosynthetic pigments, phenolic compounds, and protein content. As a photoprotective acclimation strategy against PAR + UVBR damage, an increase of 66.24 % in phenolic compounds was observed. Furthermore, PAR + UVBR treatment altering the levels of chlorophylls a and b, and total chlorophyll. In addition, total carotenoid contents decreased after PAR + UVBR treatment. The results strongly suggested that PAR + UVBR negatively affects the ultrastructure, morphology, photosynthetic pigments, and growth rates of leaf of
O
.
sativa
and, in the long term, it could affect the viability of this economically important plant.