Active membranes coated with immobilized Candida antarctica lipase B: preparation and application for continuous butyl butyrate synthesis in organic media

• Published in: Journal of membrane science Vol. 201; no. 1; pp. 55 - 64
• Main Authors: Lozano, P, Pérez-Marı́n, A.B, De Diego, T, Gómez, D, Paolucci-Jeanjean, D, Belleville, M.P, Rios, G.M, Iborra, J.L
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 31-05-2002; Elsevier
• Subjects: Active membrane; Candida antarctica lipase B; Chemical Sciences; Enzyme immobilization; Ester synthesis; Unconventional media; Candida antarctica lipase B; Ester synthesis; Unconventional media; Enzyme immobilization; Active membrane
• ISSN: 0376-7388; 1873-3123
• DOI: 10.1016/S0376-7388(01)00703-7

A method for preparing dynamic membranes by the cross-flow filtration of two water-soluble polymers (gelatin and/or polyethyleneimine, PEI) on an α-alumina ceramic support, followed by covalent attachment of
Candida antarctica lipase B (CALB) has been optimized. The obtained derivatives were tested for different immobilization parameters and selected as a function of the operational stability for the continuous hydrolysis of
p-nitrophenyl palmitate (PNPP). The gelatin–PEI active membrane provided the best results (93% coupling efficiency, 4×10
−3
U
cm
−2 activity and 22
h half-life time). This enzymatic membrane was also used as catalyst for the continuous kinetically controlled synthesis of butyl butyrate from vinyl butyrate and 1-butanol in three different organic solvents (hexane, acetone and acetonitrile) at low water content (<3% v/v). A clear enhancement in the operational stability of the enzymatic dynamic membranes was observed in these anhydrous media. The lipase-membrane derivative prepared with gelatin–PEI showed 28×10
−3
U
cm
−2 synthetic activity and a 203 day half-life time in hexane media.