Effects of dietary Glycyrrhiza polysaccharide supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets

• Published in: Animal biotechnology Vol. 34; no. 7; pp. 2273 - 2284
• Main Authors: Zhang, Cai, Li, Chenxu, Zhao, Pengli, Shao, Qi, Ma, Yanbo, Bai, Dongying, Liao, Chengshui, He, Lei, Huang, Shucheng, Wang, Xueying
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01-12-2023; Taylor & Francis Ltd
• Subjects: Antioxidants; Cecum; Community composition; Diet; Dietary supplements; GA-binding protein; Glycyrrhiza; Glycyrrhiza polysaccharide; immunity; Interleukin 10; intestinal health; Intestine; Microbiota; Microorganisms; Mucosa; piglets; Polysaccharides; Relative abundance; Superoxide dismutase; Swine; Transforming growth factor-b; Zonula occludens-1 protein
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2022.2086878

The aim of this study was to evaluate the effects of dietary Glycyrrhiza polysaccharide (GCP) supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets. One hundred and twenty 28-day-old weaned piglets were randomly assigned into five groups (four replicates per group) and fed a basal diet with GCP at 0, 500, 1000, 2000 and 4000 mg/kg for four weeks, respectively. Results showed that 1000 mg/kg GCP improved piglets' ADG and ADFI and reduced FCR (p < .05). Thus, the 0 and 1000 mg/kg GCP dose were selected for subsequent experiments. We found that 1000 mg/GCP increased SOD and T-AOC and decreased MDA in the jejunal mucosa (p < .05). Dietary 1000 mg/kg GCP also resulted in high levels of sIgA, IL-10 and TGF-β, whereas IL-2 dropped dramatically (p < .05). The relative expression levels of ZO-1, CLDN, OCLDN, TLR-4, IL-10, TGF-β, Nrf-2, SOD1 and CAT increased in the jejunal mucosa, whereas INF-γ decreased (p < .05). 1000 mg/kg GCP treatment altered the diversity and community composition of cecal microbiota in pigs, with increasing relative abundance of Bacteroidota and Lactobacillus at phylum and genus levels (p < .05), respectively. The results suggested that dietary 1000 mg/kg GCP could improve growth performance and intestinal health of weaned piglets.