Characterization of monocyte-derived dendritic cells used in immunotherapy for HIV-1-infected individuals

• Published in: Immunotherapy Vol. 10; no. 10; pp. 871 - 885
• Main Authors: da Silva, Laís Teodoro, da Silva, Wanessa Cardoso, de Almeida, Alexandre, da Silva Reis, Denise, Santillo, Bruna Tereso, Rigato, Paula Ordonhez, da Silva Duarte, Alberto José, Oshiro, Telma Miyuki
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01-08-2018; 01-08-2018
• Subjects: Adult; AIDS Vaccines - immunology; Antigens; Antiretroviral drugs; Antiviral agents; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; Cell adhesion & migration; Cell culture; Cytokines; Dendritic cells; Dendritic Cells - immunology; Drug dosages; Drug therapy; Female; HIV; HIV Infections - immunology; HIV-1; HIV/AIDS; Human immunodeficiency virus; Humans; Immune response (cell-mediated); Immunotherapy; Immunotherapy - methods; In Vitro Techniques; Infections; Lymphocytes; Lymphocytes T; Male; Middle Aged; Monocytes; Monocytes - cytology; Monocytes - immunology; Patients; Phagocytosis; Vaccines; Young Adult; Brazil; United States > US; HIV/AIDS; dendritic cells; immunotherapy
• ISSN: 1750-743X; 1750-7448
• DOI: 10.2217/imt-2017-0165

A therapeutic vaccine based on monocyte-derived dendritic cells (MDDCs) has been shown to represent a promising strategy for the treatment of cancer and viral infections. Here, we characterized the MDDCs used as an immunogen in a clinical trial for an anti-HIV-1 therapeutic vaccine.
Monocytes obtained from 17 HIV-infected individuals were differentiated into MDDCs and, after loading with autologous HIV, the cells were characterized concerning surface molecule expression, migratory and phagocytosis capacity, cytokine production and the induction of an effective cell-mediated immune response.
The MDDCs were able to induce antigen-specific responses in autologous CD4+ and CD8+ T lymphocytes.
Despite a large interindividual variability, the results suggested that MDDCs present the potential to promote immune responses in vaccinated patients.