Detoxification of high concentrations of trinitrotoluene by bacteria

• Published in: Applied biochemistry and microbiology Vol. 48; no. 1; pp. 21 - 27
• Main Authors: Solyanikova, I. P, Baskunov, B. P, Baboshin, M. A, Saralov, A. I, Golovleva, L. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 2012; SP MAIK Nauka/Interperiodica; Springer Nature B.V
• Subjects: acetates; Acinetobacter; Aromatic compounds; Bacteria; Biochemistry; Biomedical and Life Sciences; Detoxification; Kocuria palustris; Kocuria rosea; Life Sciences; Medical Microbiology; Microbiology; Pseudomonas; Soil contaminants; Soil microorganisms; trinitrotoluene; Cell Free Extract; Kocuria; Thin Layer Chroma; Dinitro; Apply Biochemistry
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683812010152

The ability of the strains-destructors of various aromatic compounds to utilize trinitrotoluene (TNT) up to concentration of 70 mg/l was shown. An increase in the TNT concentration from 100 to 150 mg/l did not inhibit its conversion rate by the Kocuria palustris RS32 strain. The Acinetobacter sp. VT11 strain utilized TNT as a sole substrate for growth; 3,5-dinitro-4-methyl anilide acetate and 2,6-dinitro-4-aminotoluene were identified as intermediates of TNT degradation by active strains of Pseudomonas sp. VT-7W and Kocuria rosea RS51. At the same time, 4-methyl-3,5-dinitroformamide was discovered for the first time upon the TNT destruction by the bacteria strains of Rhdococcus opacus 1G and Rhdococcus sp. VT-7. The active bacterial strains achieved an 82-90% destruction of TNT when they were introduced into the soil.