Over-production of human interferon-γ by HCDC of recombinant Escherichia coli

• Published in: Process biochemistry (1991) Vol. 42; no. 1; pp. 112 - 117
• Main Authors: Babaeipour, V., Shojaosadati, S.A., Robatjazi, S.M., Khalilzadeh, R., Maghsoudi, N.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2007
• Subjects: Escherichia coli; Fed-batch; Feeding strategy; High cell density cultivation (HCDC); Human interferon-γ; Specific growth rate; Feeding strategy; High cell density cultivation (HCDC); Human interferon-γ; Fed-batch; Escherichia coli; Specific growth rate
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2006.07.009

A simple fed-batch process was developed using a modified variable specific growth rate feeding strategy for high cell density cultivation of
Escherichia coli BL21 (DE3) expressing human interferon-gamma (hIFN-γ). The feeding rate was adjusted to achieve the maximum attainable specific growth rate during fed-batch cultivation. In this method, specific growth rate was changed from a maximum value of 0.55
h
−1 at the beginning of feeding and then it was reduced to 0.4
h
−1 at induction time.
The final concentration of biomass and IFN-γ was reached to ∼115
g
l
−1 (DCW) and 42.5
g(hIFN-γ)
l
−1 after 16.5
h, also the final specific yield and overall productivity of recombinant hIFN-γ (rhIFN-γ) were obtained 0.37
g(hIFN-γ)
g
−1 DCW and 2.57
g(hIFN-γ)
l
−1
h
−1, respectively. According to available data this is the highest specific yield and productivity that has been reported for recombinant proteins production yet.