A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro

• Published in: Archives of gynecology and obstetrics Vol. 280; no. 6; pp. 961 - 969
• Main Authors: Baston-Büst, Dunja M., Hess, Alexandra P., Hirchenhain, Jens, Krücken, Jürgen, Wunderlich, Frank, Krüssel, Jan S., Friebe-Hoffmann, Ulrike K.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-12-2009; Springer Nature B.V
• Subjects: Adult; Blotting, Western; Cyclooxygenase 1 - biosynthesis; Cyclooxygenase 1 - genetics; Cyclooxygenase 1 - metabolism; Cyclooxygenase 2 - biosynthesis; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Decidua - cytology; Decidua - drug effects; Decidua - enzymology; Decidua - physiology; Dinoprostone - metabolism; Endocrinology; Enzyme Activation - drug effects; Enzyme-Linked Immunosorbent Assay; Female; Gynecology; Human Genetics; Humans; Immunohistochemistry; Medicine; Medicine & Public Health; Myometrium - drug effects; Myometrium - enzymology; Myometrium - physiology; Obstetric Labor, Premature - prevention & control; Obstetrics/Perinatology/Midwifery; Original Article; Pregnancy; Relaxin - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Uterine Contraction - drug effects; Young Adult; Insulin-like growth factor family (IGF); Labour; Preterm birth; Cyclooxygenases (COX); Contraction; Relaxin (RLX)
• ISSN: 0932-0067; 1432-0711
• DOI: 10.1007/s00404-009-1046-8

Purpose
Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE
2
in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.
Methods
In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.
Results
We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.
Conclusions
These effects might potentially contribute to birth-associated induction of contractions in vivo.