Protective effects of genistein on cytochrome P-450 and vitellogenin expression in liver of zebrafish after PCB-126 exposure

• Published in: The Science of the total environment Vol. 674; pp. 71 - 76
• Main Authors: Licata, Patrizia, Piccione, Giuseppe, Fazio, Francesco, Lauriano, Eugenia Rita, Calò, Margherita
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-07-2019
• Subjects: CYP1A1; Danio rerio; Immunoreactivity; PCB-126; Vitellogenin; CYP1A1; PCB-126; Immunoreactivity; Vitellogenin; Danio rerio
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2019.03.467

The objective of the research is to study the action of Vitellogenin and P-4501A1 following coexposure at different times to genistein and PCB-126 using zebrafish as a model system. Polychlorinated biphenyls are ubiquitous substances in environment. The genistein is a phytoestrogen extracted from soybeans and it's contained in food for humans and animals. For this study, 200 adult zebrafish were used. Our findings show a marked immunoreactivity of Vtg at 12h in liver than the control with only PCB-126. Regarding effects of PCB-126 on Vtg after pretreatment with genistein in fishes, the immunohistochemistry results show a minor increase at 12h. After 24h the immunoreactivity is lower than 12h and then slightly increased at 72h with only PCB-126 and PCB-126 and genistein together. CYP1A1 progressively increases from 12h to 72h in all groups with minor immunoreactivity when we treated fish with genistein and PCB-126. We show a reduction in the estrogenic effect when the fishes were treated with genistein and PCB-126 together at 12h than the group treated with only PCB-126. Moreover, low concentrations of genistein decrease the marked P450 expression induced by PCB-126. This shows that genistein decreases the expression of P450 target genes mediated by AhR.
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•Action of Vtg and P-4501A1 following coexposure to genistein and PCB-126 in zebrafish•A marked immunoreactivity of Vtg at 12h in liver than the control with only PCB-126•Reduction in the estrogenic effect after treatment with genistein and PCB-126 at 12h•Low levels of genistein decrease the marked P450 expression induced by PCB-126.