ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID

ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID

1. Ribosomal RNA has been prepared by extracting tissues with a phenol-cresol mixture, and ribosomal RNA can be selectively precipitated with m-cresol. No rapidly labelled RNA was associated with this material. 2. However, if RNA and DNA are extracted with 4-aminosalicylate and phenol-cresol mixture...

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Bibliographic Details
Published in:Biochemical journal Vol. 96; no. 1; pp. 266 - 269
Main Author: KIRBY, K S
Format: Journal Article
Language:English
Published: England 01-07-1965
Subjects:
Acetates
Biochemical Phenomena
Biochemistry
Centrifugation
Cresols
DNA
Liver - cytology
Old Medline
Rats
Ribosomes
RNA
RNA, Ribosomal
RNA, Transfer
CENTRIFUGATION
RNA
BIOCHEMISTRY
EXPERIMENTAL LAB STUDY
LIVER CYTOLOGY
ACETATES
RATS
RIBOSOMES
CRESOLS
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Summary:1. Ribosomal RNA has been prepared by extracting tissues with a phenol-cresol mixture, and ribosomal RNA can be selectively precipitated with m-cresol. No rapidly labelled RNA was associated with this material. 2. However, if RNA and DNA are extracted with 4-aminosalicylate and phenol-cresol mixture and the nucleic acids precipitated, DNA, glycogen and s-RNA (transfer RNA) can be extracted with 3m-sodium acetate and in this case rapidly labelled RNA remains associated with the ribosomal RNA. 3. The ribosomal RNA is stable in the presence of concentrated salt solution and, although the secondary structure is lost by heating at 70 degrees in 10mm-sodium acetate, it can be re-formed in the presence of 200mm-sodium acetate. 4. The 28s and 18s components have been separated and their base compositions determined.
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ISSN:0264-6021
1470-8728
DOI:10.1042/bj0960266