Widespread prevalence and molecular epidemiology of tet(X4) and mcr-1 harboring Escherichia coli isolated from chickens in Pakistan

• Published in: The Science of the total environment Vol. 806; no. Pt 2; p. 150689
• Main Authors: Li, Ruichao, Lu, Xiaoyu, Munir, Asim, Abdullah, Sabahat, Liu, Yuan, Xiao, Xia, Wang, Zhiqiang, Mohsin, Mashkoor
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2022
• Subjects: Animals; Chickens; Co-transfer frequency; Coexistence; Escherichia coli; Escherichia coli - genetics; Escherichia coli Proteins - genetics; mcr-1; Molecular Epidemiology; Pakistan - epidemiology; Prevalence; tet(X4); Pakistan; Co-transfer frequency; tet(X4); Coexistence; Escherichia coli; Chickens; mcr-1
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.150689

The emergence and spread of plasmid-mediated tigecycline resistance gene tet(X4) and colistin resistance gene mcr-1 in Escherichia coli (E. coli) pose a potential threat to public health, due to the importance of colistin and tigecycline for treating serious clinical infections. However, the characterization of bacteria coharboring both genes was few reported. Here, we described the molecular epidemiology of tet(X4) and mcr-1 harboring E. coli strains of chicken origin in Pakistan, with methods including PCR, antimicrobial susceptibility testing, DNA transfer assays, plasmid replicon typing, whole-genome sequencing and bioinformatics analysis. The tet(X4) gene was identified in 36 isolates exhibiting high levels of tigecycline resistance (MICs, 16–128 mg/L). Worryingly, 24 of the 36 tet(X4)-bearing isolates were confirmed as colistin resistance, positive for plasmid-borne mcr-1. We observed the prevalence of tet(X4)-bearing IncFII plasmid with mcr-1-bearing IncI2 plasmid in 12 E. coli isolates, with a high co-transfer frequency except for one strain PK8233, in which tet(X4)- and mcr-1-bearing plasmids were non-transferable. Coexistence of tet(X4)-bearing IncFII plasmid with mcr-1-carrying multidrug-resistant (MDR) IncHI2 plasmid was also identified in 10 E. coli isolates, and a relatively low co-transfer frequency was obtained except PK8575, in which mcr-1 was non-transferable. The transferability of pPK8275-tetX in PK8275 and pPK8233-tetX in PK8233, that could transfer from E. coli J53 to C600 by conjugation, was interfered by certain factors in PK8275 and PK8233. This may provide new insights to prevent and control the spread of antibiotic resistance genes. Two strains were reported to co-carry tet(X4)-positive IncQ1 plasmid and mcr-1-positive IncI2 plasmid. Convergence of tet(X4) and mcr-1 genes in E. coli by conjugative or mobilizable plasmids may lead to potentially widespread transmission of such resistance genes, which may incur antibiotic-resistance crisis globally.
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•First comprehensive characterization of E. coli isolates co-carrying tet(X4) and mcr-1 outside of China•High co-transfer frequencies of tet(X4) and mcr-1 were observed.•The tet(X4) and mcr-1 harboring E. coli isolates were prevalent in food animals in Pakistan.•Plasmids were major contributors to transmission of the two vital resistance genes in different bacterial clones.