The extreme plant‐growth‐promoting properties of Pantoea phytobeneficialis MSR2 revealed by functional and genomic analysis

• Published in: Environmental microbiology Vol. 22; no. 4; pp. 1341 - 1355
• Main Authors: Nascimento, Francisco X., Hernandez, Anabel G., Glick, Bernard R., Rossi, Márcio J.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-04-2020; Wiley Subscription Services, Inc
• Subjects: Biosynthesis; Carboxylic acids; Cell walls; Colonization; Cytokinins; Evolution; Exudates; Exudation; Genes; Genome, Plant; Genomes; Genomic analysis; Indoleacetic acid; Indoleacetic Acids; Jasmonic acid; Lignin; Metabolism; Microbiological strains; Nitrogen Fixation; Nitrogenation; Pantoea; Pantoea - genetics; Pantoea - physiology; Phosphates; Phosphates - metabolism; Plant Development; Plant growth; Plant Growth Regulators - physiology; Plant growth substances; Plant roots; Plant Roots - microbiology; Plasmids; Promotion; Properties; Solubilization; Strains (organisms)
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.14946

Summary
Numerous Pantoea strains are important because of the benefit they provide in the facilitation of plant growth. However, Pantoea have a high level of genotypic diversity and not much is understood regarding their ability to function in a plant beneficial manner. In the work reported here, the plant growth promotion activities and the genomic properties of the unusual Pantoea phytobeneficialis MSR2 are elaborated, emphasizing the genetic mechanisms involved in plant colonization and growth promotion. Detailed analysis revealed that strain MSR2 belongs to a rare group of Pantoea strains possessing an astonishing number of plant growth promotion genes, including those involved in nitrogen fixation, phosphate solubilization, 1‐aminocyclopropane‐1‐carboxylic acid deaminase activity, indoleacetic acid and cytokinin biosynthesis, and jasmonic acid metabolism. Moreover, the genome of this bacterium also contains genes involved in the metabolism of lignin and other plant cell wall compounds, quorum‐sensing mechanisms, metabolism of plant root exudates, bacterial attachment to plant surfaces and resistance to plant defences. Importantly, the analysis revealed that most of these genes are present on accessory plasmids that are found within a small subset of Pantoea genomes, reinforcing the idea that Pantoea evolution is largely mediated by plasmids, providing new insights into the evolution of beneficial plant‐associated Pantoea.