Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Bothrops erythromelas Snake Venom

• Published in: Toxins Vol. 12; no. 9; p. 606
• Main Authors: Nunes, Ellynes, Frihling, Breno, Barros, Elizângela, de Oliveira, Caio, Verbisck, Newton, Flores, Taylla, de Freitas Júnior, Augusto, Franco, Octávio, de Macedo, Maria, Migliolo, Ludovico, Luna, Karla
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 20-09-2020; MDPI AG
• Subjects: animal venom; antibacterial and antibiofilm activity; bacterial resistance; purification; animal venom; antibacterial and antibiofilm activity; bacterial resistance; purification
• ISSN: 2072-6651; 2072-6651
• DOI: 10.3390/toxins12090606

Bacterial resistance is a worldwide public health problem, requiring new therapeutic options. An alternative approach to this problem is the use of animal toxins isolated from snake venom, such as phospholipases A
(PLA
), which have important antimicrobial activities.
is one of the snake species in the northeast of Brazil that attracts great medical-scientific interest. Here, we aimed to purify and characterize a PLA
from
, searching for heterologous activities against bacterial biofilms.
Venom extraction and quantification were followed by reverse-phase high-performance liquid chromatography (RP-HPLC) in C18 column, matrix-assisted ionization time-of-flight (MALDI-ToF) mass spectrometry, and sequencing by Edman degradation. All experiments were monitored by specific activity using a 4-nitro-3-(octanoyloxy) benzoic acid (4N
OBA) substrate. In addition, hemolytic tests and antibacterial tests including action against
,
and
were carried out. Moreover, tests of antibiofilm action against
were also performed.
PLA
, after one purification step, presented 31
-terminal amino acid residues and a molecular weight of 13.6564 Da, with enzymatic activity confirmed in 0.06 µM concentration. Antibacterial activity against
(IC
= 30.2 µM) and antibiofilm activity against
(IC
= 1.1 µM) were observed.
This is the first time that PLA
purified from
venom has appeared as an alternative candidate in studies of new antibacterial medicines.