Virus-Specific Messenger RNA on Free and Membrane-Bound Polyribosomes from Cells Infected with Rauscher Leukemia Virus

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 71; no. 4; pp. 1093 - 1097
• Main Authors: Gielkens, A. L. J., Salden, M. H. L., Bloemendal, H.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-04-1974; National Acad Sciences
• Subjects: Animals; Biological Sciences: Biochemistry; Bone Marrow Cells; Cell Fractionation; Cell Line; Cell lines; Centrifugation; Centrifugation, Density Gradient; Complementary DNA; DNA; DNA, Single-Stranded - metabolism; Messenger RNA; Mice; Mice, Inbred BALB C; Nucleic Acid Hybridization; Polyribosomes; Polyribosomes - analysis; Rauscher Virus; Ribosomes; RNA; RNA, Messenger - analysis; RNA, Viral - analysis; RNA, Viral - metabolism; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.71.4.1093

Cells infected by Rauscher leukemia virus synthesize virus-specific RNA which can be detected by hybridization to the single-stranded DNA copy of the viral RNA. Evidence is provided that virus-specific RNA is present in free and membrane-bound polyribosomes of these cells. The relative content of virus-specific RNA, as measured by hybridization, is 6-10 times less on free polyribosomes than on membrane-bound polyribosomes. The messenger RNA associated with both classes of polyribosomes was characterized by density gradient centrifugation. In addition to a major RNA species identified as 36S RNA, at least 2 minor components in the 14S and 21S region have also been found. There is a striking difference in the distribution of these RNA species between free and membrane-bound polyribosomes.