Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)

• Published in: Molecular ecology resources Vol. 19; no. 5; pp. 1333 - 1345
• Main Authors: Ji, Yunheng, Liu, Changkun, Yang, Zhenyan, Yang, Lifang, He, Zhengshan, Wang, Hengchang, Yang, Junbo, Yi, Tingshuang
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-09-2019
• Subjects: Araliaceae; Bar codes; Biogeography; Deoxyribonucleic acid; DNA; DNA Barcoding, Taxonomic - methods; DNA, Plant - genetics; DNA, Ribosomal - genetics; Economic conditions; Gene sequencing; Genomes; next‐generation DNA barcodes; Nucleotide sequence; Panax; Panax - classification; Panax - genetics; Phylogenetics; Phylogeny; Plastids - genetics; plastome; Plastomes; Power plants; Resolution; Ribosomal DNA; Sequence Analysis, DNA - methods; Speciation; Species; species discrimination; standard DNA barcodes; Panax; next-generation DNA barcodes; plastome; species discrimination; standard DNA barcodes; ribosomal DNA
• ISSN: 1755-098X; 1755-0998
• DOI: 10.1111/1755-0998.13050

Complete plastid genome (plastome) sequences and nuclear ribosomal DNA (nrDNA) regions have been proposed as candidates for the next generation of DNA barcodes for plant species discrimination. However, the efficacy of this approach still lacks comprehensive evaluation. We carried out a case study in the economically important but phylogenetically and taxonomically difficult genus Panax (Araliaceae). We generated a large data set of plastomes and nrDNA sequences from multiple accessions per species. Our data improved the phylogenetic resolution and levels of species discrimination in Panax, compared to any previous studies using standard DNA barcodes. This provides new insights into the speciation, lineage diversification and biogeography of the genus. However, both plastome and nrDNA failed to completely resolve the phylogenetic relationships in the Panax bipinnatifidus species complex, and only half of the species within it were recovered as monophyletic units. The results suggest that complete plastome and ribosomal DNA sequences can substantially increase species discriminatory power in plants, but they are not powerful enough to fully resolve phylogenetic relationships and discriminate all species, particularly in evolutionarily young and complex plant groups. To gain further resolving power for closely related species, the addition of substantial numbers of nuclear markers is likely to be required.