Resveratrol-Enriched Rice Callus Extract Inhibits Oxidative and Cellular Melanogenic Activities in Melan-A Cells

• Published in: Antioxidants Vol. 13; no. 6; p. 625
• Main Authors: Monmai, Chaiwat, Kim, Jin-Suk, Baek, So-Hyeon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-05-2024; MDPI
• Subjects: AKT protein; Antioxidants; Ascorbic acid; Callus; Cell activation; Cell culture; Cell size; Dopachrome isomerase; Experiments; Extracellular signal-regulated kinase; Hyperpigmentation; Kinases; Melanin; Microphthalmia-associated transcription factor; MITF; Potassium; Proteins; Resveratrol; Rice; Seeds; Skin; Skin diseases; transgenic rice; tyrosinase; Vitamin C; St Louis Missouri; United States > US; South Korea; hyperpigmentation; MITF; tyrosinase; resveratrol; transgenic rice
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox13060625

The excessive production of melanin can cause skin diseases and hyperpigmentation. In this study, resveratrol contained in Dongjin rice seed (DJ526) was increased through callus induction. The antioxidant capacity of resveratrol-enriched rice callus was evaluated using the ABTS radical scavenging method and was equivalent to that of vitamin C. DJ526 rice callus extract significantly increased antioxidant activities in a concentration-dependent manner. The anti-melanogenesis effects of DJ526 rice callus extract were also evaluated in melan-a cells. Resveratrol-enriched rice callus extract significantly (i) decreased the size and number of melanin-containing cells, (ii) suppressed the activity of cellular tyrosinase and melanin content, (iii) downregulated the expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2, (iv) increased the expression of phosphorylated extracellular signal-regulated kinase 1/2 and protein kinase B, and (v) inhibited the activation of phosphorylated p38 in melan-a cells. From the above observations, DJ526 rice callus extract showed strong antioxidant and anti-melanogenesis activity at the concentration test. These findings indicate the potential of resveratrol-enriched rice callus as a novel agent for controlling hyperpigmentation.