Neuropeptide Y modulates growth hormone but not luteinizing hormone secretion from prepuberal gilt anterior pituitary cells in culture

Pituitary cells, from seven 160- to 170-day-old pigs, were studied in primary culture to determine the affects NPY on LH and GH secretion at the level of the pituitary. On day 4 of culture, medium was discarded, plates were rinsed twice with serum-free medium and cells were cultured in 1 ml fresh me...

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Published in:	Domestic animal endocrinology Vol. 29; no. 3; pp. 548 - 555
Main Authors:	Barb, C.R., Barrett, J.B.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-10-2005
Subjects:	Analysis of Variance Animals Cells, Cultured Dose-Response Relationship, Drug Drug Interactions Female Growth hormone Growth Hormone - secretion Growth Hormone-Releasing Hormone - administration & dosage Growth Hormone-Releasing Hormone - physiology Luteinizing hormone Luteinizing Hormone - secretion Neuropeptide Y Neuropeptide Y - administration & dosage Neuropeptide Y - physiology Peptide Fragments - administration & dosage Peptide Fragments - physiology Pig Pituitary Pituitary Gland, Anterior - cytology Pituitary Gland, Anterior - secretion Sexual Maturation - physiology Swine Neuropeptide Y Luteinizing hormone Growth hormone Pituitary Pig
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Summary:	Pituitary cells, from seven 160- to 170-day-old pigs, were studied in primary culture to determine the affects NPY on LH and GH secretion at the level of the pituitary. On day 4 of culture, medium was discarded, plates were rinsed twice with serum-free medium and cells were cultured in 1 ml fresh medium without serum and challenged individually with 10 −10, 10 −8 or 10 −6 M [Ala 15]-h growth hormone-releasing factor-(1–29)NH 2 (GRF); 10 −9, 10 −8 or 10 −7 M GnRH or 10 −9, 10 −8, 10 −7 or 10 −6 M NPY individually or in combinations with 10 −9 or 10 −8 M GnRH or 10 −8 or 10 −6 M GRF. Cells were exposed to treatment for 4 h at which time medium was harvested and quantified for LH and GH. Basal LH secretion (control; n = 7 pituitaries) was 12 ± 6 ng/well. Relative to control at 4 h, 10 −9, 10 −8 and 10 −7 M GnRH increased ( P < 0.01) LH secretion by 169, 176 and 197%, respectively. Neuropeptide-Y did not alter ( P > 0.4) basal LH secretion nor 10 −8 M GnRH-induced increase in LH secretion but 10 −9 M GnRH-stimulated LH secretion was reduced by NPY and was not different from control or GnRH alone. Basal GH secretion (control; n = 7 pituitaries) was 56 ± 12 ng/well. Relative to control at 4 h, 10 −10, 10 −8 and 10 −6 M GRF increased GH secretion by 111%, 125% ( P < 0.01) and 150% ( P < 0.01), respectively. Only 10 −6 M (134%) and 10 −7 M (125%) NPY increased ( P < 0.04) basal GH secretion. Addition of 10 −9, 10 −8 and 10 −7 M NPY in combination with 10 −8 M GRF suppressed ( P < 0.04) GRF-stimulated GH secretion. However, 10 −9 M NPY enhanced ( P < 0.06) the GH response to 10 −6 M GRF. These results demonstrate that NPY may directly modulate GH secretion at the level of the pituitary gland.
ISSN:	0739-7240 1879-0054
DOI:	10.1016/j.domaniend.2005.03.004