Intragenic Conflict in Phylogenomic Data Sets

• Published in: Molecular biology and evolution Vol. 37; no. 11; pp. 3380 - 3388
• Main Authors: Smith, Stephen A, Walker-Hale, Nathanael, Walker, Joseph F
• Format: Journal Article
• Language: English
• Published: United States 01-11-2020
• Subjects: Animals; Computer Simulation; Datasets as Topic; Insecta - genetics; Mammals - genetics; Models, Genetic; Phylogeny; intragenic conflict; phylogenomics; recombination
• ISSN: 0737-4038; 1537-1719
• DOI: 10.1093/molbev/msaa170

Most phylogenetic analyses assume that a single evolutionary history underlies one gene. However, both biological processes and errors can cause intragenic conflict. The extent to which this conflict is present in empirical data sets is not well documented, but if common, could have far-reaching implications for phylogenetic analyses. We examined several large phylogenomic data sets from diverse taxa using a fast and simple method to identify well-supported intragenic conflict. We found conflict to be highly variable between data sets, from 1% to >92% of genes investigated. We analyzed four exemplar genes in detail and analyzed simulated data under several scenarios. Our results suggest that alignment error may be one major source of conflict, but other conflicts remain unexplained and may represent biological signal or other errors. Whether as part of data analysis pipelines or to explore biologically processes, analyses of within-gene phylogenetic signal should become common.