Neutrophil kinetics in the dog

• Published in: The Journal of clinical investigation Vol. 55; no. 4; pp. 833 - 839
• Main Authors: Deubelbeiss, K A, Dancey, J T, Harker, L A, Finch, C A
• Format: Journal Article
• Language: English
• Published: United States 01-04-1975
• Subjects: Animals; Bone Marrow Cells; Dogs; Erythrocytes - metabolism; Female; Granulocytes - metabolism; Isoflurophate - metabolism; Leukocyte Count; Male; Mitosis; Neutrophils - metabolism; Neutrophils - physiology; Phosphorus Radioisotopes; Thymidine - metabolism; Time Factors; Tritium
• ISSN: 0021-9738
• DOI: 10.1172/JCI107994

The production of neutrophils in dogs has been estimated from the number of postmitotic neutrophils in the marrow and the transit time of a [3H]-thymidine pulse. The number of postmitotic neutrophils was derived from the erythron iron turnover measurement of erythroid number and the neutrophil-erythroid ratio in bone marrow sections. The mean value for marrow postmitotic neutrophils in dogs was 5.61 plus or minus 0.56 times 10-9 cells/kg. The mean transit time of these neutrophils was calculated to be 82.1 h. A marrow production of 1.65 times 10-9 neutrophils/kg/day was calculated from these data. The turnover of circulating neutrophils was measured by [3H]thymidine and [32P]diisopropylphospho-fluoridate (DF32P) labeling of blood neutrophils. [3H]-Thymidine labeling gave a calculated recovery of 65 per cent, a t1/2 disappearance time of 6.7 h, and a calculated turnover of 1.66 times 10-9 cells/kg/day. Corresponding results with DF32P tagging were 51 per cent, 5.4 h, and 2.89 times 10-9 cells/kg/day. The discrepancy between these two tags persisted in doubly tagged cells and was considered to be due to elution of DF32P.