Heteroglycan of an edible mushroom Pleurotus cystidiosus: Structural characterization and study of biological activities

• Published in: International journal of biological macromolecules Vol. 95; pp. 833 - 842
• Main Authors: Panda, Bibhash C., Maity, Prasenjit, Nandi, Ashis K., Pattanayak, Manabendra, Manna, Dilip K., Mondal, Soumitra, Tripathy, Satyajit, Roy, Somenath, Acharya, Krishnendu, Islam, Syed S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2017
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antioxidants - toxicity; Biological activities; Carbohydrate Sequence; Dose-Response Relationship, Drug; Humans; Lymphocytes - cytology; Lymphocytes - drug effects; NMR studies; Pleurotus - chemistry; Pleurotus cystidiosus; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Polysaccharides - toxicity; Water - chemistry; Pleurotus cystidiosus; Biological activities; NMR studies
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.11.121

•Polysaccharide of an edible mushroom Pleurotus cystidiosus.•Structure determination by chemical and 1D/2D NMR analysis.•Biological activities of the heteroglycan of Pleurotus cystidiosus.
A water soluble heteroglycan (PCPS) was isolated from the aqueous extract of an edible mushroom Pleurotus cystidiosus. Structural characterization of the heteroglycan was carried out using total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and 1D/2D NMR experiments. Sugar analysis indicated the presence of glucose, galactose, and mannose in a molar ratio of nearly 6:2:1 respectively. The chemical and NMR analysis of the PCPS indicated the presence of a repeating unit with a backbone consisting of one unit of (1→6)-β-d-glucopyranosyl, two (1→3)-β-d-glucopyranosyl, one (1→3)-α-d-glucopyranosyl, one (1→6)-α-d-glucopyranosyl, and two (1→6)-α-d-galactopyranosyl moieties respectively, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 with terminal β-d-glucopyranosyl and another (1→6)-α-d-galactopyranosyl residue was branched at O-2 with terminal β-d-mannopyranosyl moiety. The polysaccharide was found to exhibit cellular activities at different concentrations (10, 25, 50, 100, 200, 400μg/mL) and maintained the redox balance as well as reduced lipid per oxidation which protect the cell destruction.