Basella alba stem extract integrated poly (vinyl alcohol)/chitosan composite films: A promising bio-material for wound healing

• Published in: International journal of biological macromolecules Vol. 225; pp. 673 - 686
• Main Authors: D'souza, Oshin Jacintha, Gasti, Tilak, Hiremani, Vishram D., Pinto, Jennifer P., Contractor, Shafa S., Shettar, Arun K., Olivia, Diana, Arakera, Suresh B., Masti, Saraswati P., Chougale, Ravindra B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-01-2023
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-inflammatory; Basella alba stem extract; Chitosan; Cytotoxicity; Escherichia coli; Humans; Plant Extracts - pharmacology; Polyvinyl Alcohol; PVA; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Wound Healing; Basella alba stem extract; Wound healing; PC; BAE; Cytotoxicity; PVA; Chitosan; Anti-inflammatory; PCB
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.11.130

Natural extract-based bio-composite material for wound healing is gaining much attention due to risk of infection and high cost of commercial wound dressing film causes serious problem on the human well-being. Herein, the study outlines the preparation of Poly (vinyl alcohol)/Chitosan/Basella alba stem extract (BAE) based bio-composite film through solvent casting technique and well characterized for wound healing application. Incorporation of BAE into Poly (vinyl alcohol)/Chitosan matrix has shown existence of secondary interactions confirmed by FT-IR analysis. Good morphology, thermal stability and significant improvement in flexibility (∼63.38 %) of the films were confirmed by SEM, TGA and Mechanical test results, respectively. Hydrophilic property (∼9.04 %), water vapor transmission rate (∼70.07 %), swelling ability (∼14.7 %) and degradation rate (∼14.04 %) were enhanced with increase in BAE content. In-vitro studies have shown good antibacterial activity against foremost infectious bacterial strains S. aureus and E. coli. Additionally, BAE integrated Poly (vinyl alcohol)/Chitosan film has amplified anti-inflammatory (∼79.38 %) property, hemocompatibility and excellent biocompatibility (94.9 %) was displayed by cytotoxicity results. Moreover, in-vitro scratch assay and cell adhesion test results illustrated prominent wound healing (96.5 %) and adhesion. Overall results of the present work proclaim that developed bio-composite film could be utilized as a biomaterial in wound care applications.
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