Strip-dried blood sampling: applicability for bovine leukemia virus detection with ELISA and real-time PCR

• Published in: Journal of virological methods Vol. 263; pp. 101 - 104
• Main Authors: Saushkin, Nikolay Yu, Samsonova, Jeanne V., Osipov, Alexander P., Kondakov, Sergey E.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2019
• Subjects: Animals; Antibodies, Viral - blood; Blood Specimen Collection - instrumentation; Blood Specimen Collection - veterinary; Bovine leukemia virus; Cattle; ELISA; Enzootic Bovine Leukosis - blood; Enzootic Bovine Leukosis - diagnosis; Enzyme-Linked Immunosorbent Assay - veterinary; Leukemia Virus, Bovine - isolation & purification; Real-time PCR; Real-Time Polymerase Chain Reaction - veterinary; RNA, Viral - blood; RNA, Viral - genetics; Strip-dried blood; Temperature; Real-time PCR; Strip-dried blood; Bovine leukemia virus; ELISA
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2018.11.004

•Original approach for BLV detection with ELISA and real-time PCR in strip-dried blood was proposed.•Concordance between the results of ELISA and real-time PCR was 93.4% (n = 334).•Even distribution of the analyzed components along the sampling strip was demonstrated.•Storage stability of proviral DNA and antibodies against BLV in strip-dried samples was shown.
We recently proposed a new so-called strip-dried format aimed for convenient use of dried biomaterial in diagnostic purposes. In this work, 334 blood samples obtained in strip-dried form were used for bovine leucosis analysis with ELISA and real-time PCR methods. High percentage of seropositive animals (18.3%) let us estimate both indirect (serological) and direct methods applicability for the analysis of strip-dried blood samples and also to compare them (PCR results concurred with ELISA in 93.4% cases). Parallel analysis of native and corresponding strip-dried samples approved the proposed format as a reliable analytical way of sampling being in 100% concordance with conventional serum/whole blood ELISA and PCR analysis. Even distribution of antibodies against bovine leukemia virus along the membrane carrier was demonstrated by square-to-square analyzing of the sample strip (CV not exceeded 7%). Also, strip-dried blood samples showed enhanced stability at elevated temperatures comparing to liquid serum. The proposed strip-blood format is a promising way of sampling, storage and transportation and can find application in veterinary practice for infectious disease monitoring.