Nitric oxide effects on Rhodnius prolixus’s immune responses, gut microbiota and Trypanosoma cruzi development

• Published in: Journal of insect physiology Vol. 126; p. 104100
• Main Authors: Batista, Kate Katherine da Silva, Vieira, Cecília Stahl, Florentino, Emmanuelle Batista, Caruso, Karina Francine Bravo, Teixeira, Paula Thais Pinheiro, Moraes, Caroline da Silva, Genta, Fernando Ariel, de Azambuja, Patrícia, de Castro, Daniele Pereira
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-2020
• Subjects: Animals; Antibacterial activity; Arginine - antagonists & inhibitors; Arginine - pharmacology; Catalase; Catalase - drug effects; Catalase - metabolism; Gastrointestinal Microbiome - drug effects; Gastrointestinal Tract - drug effects; Gastrointestinal Tract - microbiology; Gene Expression - drug effects; Genes, Insect; Hemolymph - drug effects; Hemolymph - immunology; Hemolymph - metabolism; Immune System - drug effects; Immunity, Humoral - drug effects; Insect Vectors - immunology; Insect Vectors - microbiology; Insect Vectors - parasitology; Monophenol Monooxygenase - drug effects; Monophenol Monooxygenase - metabolism; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide - metabolism; Nitric Oxide - pharmacology; Nitric oxide synthase; Nitric Oxide Synthase - genetics; Nitric Oxide Synthase - metabolism; Parasite; Phenoloxidase; Rhodnius - immunology; Rhodnius - microbiology; Rhodnius - parasitology; Triatomine; Trypanosoma cruzi - drug effects; Phenoloxidase; Parasite; Nitric oxide synthase; Antibacterial activity; Catalase; Triatomine
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2020.104100

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•L-arginine treatment decreased the number of Trypanosoma cruzi in Rhodnius prolixus.•Rhodnius prolixus treated with L-NAME enhances Trypanosoma cruzi infection.•NO levels caused by L-arginine and L-NAME treatments affect other immune responses.•L-arginine and L-NAME treatment reduces gut microbiota of Rhodnius prolixus.
The immune system of Rhodnius prolixus comprehends the synthesis of different effectors that modulate the intestinal microbiota population and the life cycle of the parasite Trypanosoma cruzi inside the vector midgut. One of these immune responses is the production of reactive nitrogen species (RNS) derived by the action of nitric oxide synthase (NOS). Therefore, we investigated the effects of L-arginine, the substrate for nitric oxide (NO) production and Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of NOS, added in the insect blood meal. We analyzed the impact of these treatments on the immune responses and development of intestinal bacteria and parasites on R. prolixus nymphs. The L-arginine treatment in R. prolixus nymphs induced a higher NOS gene expression in the fat body and increased NO production, but reduced catalase and antimicrobial activities in the midgut. As expected, L-NAME treatment reduced NOS gene expression in the fat body. In addition, L-NAME treatment diminished catalase activity in the hemolymph and posterior midgut reduced phenoloxidase activity in the anterior midgut and increased the antimicrobial activity in the hemolymph. Both treatments caused a reduction in the cultivatable intestinal microbiota, especially in insects treated with L-NAME. However, T. cruzi development in the insect’s digestive tract was suppressed after L-arginine treatment and the opposite was observed with L-NAME, which resulted in higher parasite counts. Therefore, we conclude that induction and inhibition of NOS and NO production are associated with other R. prolixus humoral immune responses, such as catalase, phenoloxidase, and antibacterial activities in different insect organs. These alterations reflect on intestinal microbiota and T. cruzi development.