An insight into differentially regulated genes in resistant and susceptible genotypes of potato in response to tomato leaf curl New Delhi virus-[potato] infection

• Published in: Virus research Vol. 232; pp. 22 - 33
• Main Authors: Jeevalatha, Arjunan, Siddappa, Sundaresha, Kumar, Ashwani, Kaundal, Priyanka, Guleria, Anupama, Sharma, Sanjeev, Nagesh, Mandadi, Singh, Bir Pal
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-03-2017
• Subjects: Apical leaf curl disease; Begomovirus - pathogenicity; Begomovirus - physiology; Disease Resistance - genetics; Disease Susceptibility - immunology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Ontology; Genotype; Host-Pathogen Interactions; Microarray; Microarray Analysis; Molecular Sequence Annotation; Plant Diseases - genetics; Plant Diseases - immunology; Plant Immunity - genetics; Plant Leaves - genetics; Plant Leaves - immunology; Plant Leaves - virology; Plant Proteins - genetics; Plant Proteins - immunology; Potato; Signal Transduction; Solanum tuberosum - genetics; Solanum tuberosum - immunology; Solanum tuberosum - virology; ToLCNDV-[potato]; Viral Load; Potato; Apical leaf curl disease; Microarray; Gene expression; ToLCNDV-[potato]
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2017.01.015

•Microarray analysis was done in contrasting potato genotypes inoculated with ToLCNDV-[potato].•Analysis indicated that 1111 and 2588 genes were altered (>2 fold) in K. Bahar and K. Pukhraj, respectively.•Cell cycle genes, R genes, HSPs, F-box proteins and transcription factors were identified in K. Bahar.•Several genes with unknown functions were 5 to 100 fold changed in both cultivars.•VIGS based studies were carried out for three DEGs in Kufri Pukhraj.
Apical leaf curl disease, caused by tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) is one of the most important viral diseases of potato in India. Genetic resistance source for ToLCNDV in potato is not identified so far. However, the cultivar Kufri Bahar is known to show lowest seed degeneration even under high vector levels. Hence, microarray analysis was performed to identify differentially regulated genes during ToLCNDV-[potato] infection in a resistant (Kufri Bahar) and a susceptible cultivar (Kufri Pukhraj). Under artificial inoculation conditions, in Kufri Pukhraj, symptom expressions started at 15days after inoculation (DAI) and then progressed to severe symptoms, whereas no or only very mild symptoms were observed in Kufri Bahar up to 35 DAI. Correspondingly, qPCR assay indicated a high viral load in Kufri Pukhraj and a very low viral load in Kufri Bahar. Microarray analysis showed that a total of 1111 genes and 2588 genes were differentially regulated (|log2 (Fold Change)|>2) in Kufri Bahar and Kufri Pukhraj, respectively, following ToLCNDV-[potato] infection. Gene ontology and mapman analyses revealed that these altered transcripts were involved in various biological & metabolic processes. Several genes with unknown functions were 5 to 100 fold expressed after virus infection and further experiments are necessary to ascertain their role in disease resistance or susceptibility. This study gives an insight into differentially regulated genes in response to ToLCNDV-[potato] infection in resistant and susceptible cultivars and could serve as the basis for the development of new strategies for disease management.