Comparative evaluation of Pseudomonas species in single chamber microbial fuel cell with manganese coated cathode for reactive azo dye removal

• Published in: International biodeterioration & biodegradation Vol. 144; p. 104744
• Main Authors: Ilamathi, R., Merline Sheela, A., Nagendra Gandhi, N.
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01-10-2019; Elsevier BV
• Subjects: Azo dyes; Biochemical fuel cells; Color removal; Decoloring; Decolorization; Dye decolorization; Fuel cells; Fuel technology; Industrial applications; Manganese; Manganese coated cathode; Maximum power density; Microbial fuel cell; Microorganisms; Phenazine; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas fluorescens; Pseudomonas sp; Reactive azo dye; Single-chamber; Microbial fuel cell; Pseudomonas sp; Manganese coated cathode; Single-chamber; Reactive azo dye; Dye decolorization
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2019.104744

Microbial fuel cell (MFCs), distinguished by different strains of Pseudomonas species; Pseudomonas aeruginosa (MPEM-MFC I) and Pseudomonas fluorescens (MPEM-MFC II), was analyzed. Results have shown that, over a period of 360 h in the presence of 0.5 mM of model dye, MPEM MFC I produced the maximum power density of 2887 ± 13 μW m−2 (RO-16) and 1906 ± 7 μW m−2 (RB-5) compared with MPEM-MFC II with 1896 ± 15 μW m−2 (RO-16) and 1028 ± 9 μW m−2 (RB-5). Decolorization efficiency of MPEM-MFC I was 98 ± 1.2% (RO-16) and 95 ± 2% (RB-5). Total phenazine production in MPEM-MFC I was 12.3 ± 0.5 μg mL−1 higher than that of 8.9 ± 0.05 μg mL−1 (MPEM-MFC II) and its production have positive influence of electron shuttling that brought out high power output. Addition of phenazine externally reduced the dye degradation. Bioadhesion capability of P. aeruginosa on the anode reduced the internal resistance in MFCs. Thus the implementation of MFC is a most promising technology for the complete decolorization of reactive azo dyes and it has potential economic benefits in real-life industrial application.
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•MPEM MFC I improved the rate of azo dye decolorization (RO-16 and RB-5).•Decolourizing potential of Pseudomonas aeruginosa in MPEM MFC was due to the action of azoreductase enzyme.•Phenazine secreted by the bacteria influence as a mediator of electron transfer.•Growth of anodic electroactive microbial biofilm of P. aeruginosa improved the redox behaviour compared to P. fluorescens.