A method for the isolation of nuclear DNA from cotton (Gossypium) leaves

Cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during DNA isolation. A procedure to isolate nuclear DNA from cotton (Gossypium hirsutum L.) has been developed. The method is based on the rapid initial isolation of nuclei in a gl...

Full description

Saved in:

Bibliographic Details
Published in:	Analytical biochemistry Vol. 210; no. 1; p. 34
Main Authors:	Dabo, S M, Mitchell, Jr, E D, Melcher, U
Format:	Journal Article
Language:	English
Published:	United States 01-04-1993
Subjects:	Blotting, Southern Cell Fractionation - methods Cell Nucleus - chemistry DNA - genetics DNA - isolation & purification Electrophoresis, Agar Gel Evaluation Studies as Topic Glucose Gossypium - chemistry Gossypium - genetics Nucleic Acid Hybridization Oxidation-Reduction Plants - chemistry Plants - genetics Polymorphism, Restriction Fragment Length
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Cotton leaves contain high levels of polyphenolic compounds that irreversibly interact with proteins and nucleic acids during DNA isolation. A procedure to isolate nuclear DNA from cotton (Gossypium hirsutum L.) has been developed. The method is based on the rapid initial isolation of nuclei in a glucose medium designed to stabilize nuclear structure and composition while preventing covalent interactions with polyphenolics. The resulting DNA is high in yield and purity and is suitable for Southern-blot hybridization analysis and restriction fragment length polymorphism analysis.
ISSN:	0003-2697
DOI:	10.1006/abio.1993.1146