Effects of Pseudomonas putida WCS358r and its genetically modified phenazine producing derivative on the Fusarium population in a field experiment, as determined by 18S rDNA analysis

• Published in: Soil biology & biochemistry Vol. 34; no. 7; pp. 1021 - 1025
• Main Authors: Leeflang, P., Smit, E., Glandorf, D.C.M., van Hannen, E.J., Wernars, K.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-07-2002; New York, NY Elsevier Science
• Subjects: 18S rDNA; Agronomy. Soil science and plant productions; Amplified ribosomal DNA restriction analyses; Biochemistry and biology; Biological and medical sciences; Biological control; Chemical, physicochemical, biochemical and biological properties; Control; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fusarium; Genetically modified microorganisms; Microbiology; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Phytopathology. Animal pests. Plant and forest protection; Pseudomonas putida; Soil; Soil science; Wheat rhizosphere; Amplified ribosomal DNA restriction analyses; Fusarium; Soil; 18S rDNA; Wheat rhizosphere; Genetically modified microorganisms; Pseudomonas putida; Pseudomonadales; Monocotyledones; Plant pathogen; Biological control; Suppression; Biodiversity; Cereal crop; Field experiment; Fungi; Phenazine; Antifungal agent; Gramineae; Efficiency; Angiospermae; Interspecific competition; Bacteria; Pseudomonadaceae; Antagonist; Fungi Imperfecti; Intraspecific comparison; Thallophyta; Phylogenetic tree; Pest and disease control; Population structure; Biological activity; Rhizosphere; Genetic identification; Spermatophyta; Genetically modified organism; Triticum aestivum
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/S0038-0717(02)00035-4

We measured effects of
Pseudomonas putida WCS358r and its genetically modified phenazine producing derivative on the
Fusarium population in the soil of a wheat field in the Netherlands. We used 18S rDNA analysis to study the
Fusarium population through a strategy based on screening clone libraries by using amplified ribosomal DNA restriction analyses (ARDRA). After screening a total of 1000 clones, 70 clones had a
Fusarium-like ARDRA pattern. Phylogenetic analysis showed that 51 out of 70 of these clones cluster in a monophyletic group together with the
Fusarium isolates that were also obtained from the experimental field, suggesting that these clones originated from fungal strains belonging to the genus
Fusarium. Both the introduced
Pseudomonas parental strain and the GMM inhibit the development of the
Fusarium and probably account for the higher diversity of
Fusarium-like clone types at day 40 compared to day 13 and 27. While the antagonistic properties of
P. putida WCS358r appear to suppress the
Fusarium population, the introduced genetic modification does not seem to play a great additional role.