Coproduction of single cell protein and lipid from lignocellulose derived carbohydrates and inorganic ammonia salt with soluble ammonia recycling

• Published in: Bioresource technology Vol. 384; p. 129345
• Main Authors: Zhang, Bin, Ren, Dayu, Liu, Qi, Liu, Xiucai, Bao, Jie
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2023
• Subjects: Ammonia - metabolism; Carbohydrates; Fermentation; Lignocellulose; Lipids; Microbial lipid; Single cell protein; Sodium Chloride - metabolism; Sugars - metabolism; Trichosporon - metabolism; Trichosporon cutaneum; Wastewater; Single cell protein; Microbial lipid; Lignocellulose; Trichosporon cutaneum; Wastewater
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129345

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•SCP and lipid were coproduced from wheat straw and (NH4)2SO4 by oleaginous yeast.•Engineered Trichosporon cutaneum MP11 with excellent adaptability was employed.•The cellulosic SCP and lipid titer reached 24.4 ± 1.5 g/L and 11.8 ± 1.2 g/L at 48 h.•Residual (NH4)2SO4 in wastewater was recycled with stable fermentation performance.
Co-production of single cell protein (SCP) and lipid from lignocellulose-derived carbohydrates and inorganic ammonia offers a promising alternative for poultry or aquaculture feeds. An engineered oleaginous yeast Trichosporon cutaneum MP11 showed great potential for producing SCP and lipid from wheat straw and ammonia sulfate with minimum nutrient input. Trichosporon cutaneum MP11 showed stronger SCP and lipid fermentability using dry acid pretreated and biodetoxified wheat straw than using pure sugars. The residual ammonium sulfate in fermentation broth was recycled up to five times, resulting in ∼70% of nitrogen fixation into SCP. The overall yield of SCP and lipid from lignocellulose-derived sugars was 0.15 g/g and 0.11 g/g, respectively. This translates to the production of one ton of SCP (0.56 ton) and lipid (0.44 ton) from 6.6 tons of wheat straw, or one ton of SCP and lipid containing yeast cells (dry) from 4.8 tons of wheat straw.