Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo

• Published in: International journal of biological macromolecules Vol. 149; pp. 41 - 50
• Main Authors: Hou, Ranran, Xu, Tianli, Li, Qiu, Yang, Fengfang, Wang, Chunyuan, Huang, Tingting, Hao, Zhihui
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15-04-2020
• Subjects: Animals; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cell Line; Echinacea - chemistry; Echinacea purpurea polysaccharide; Free radical scavenging test; Mice; Mice, Inbred ICR; Oxidative damage model; Oxidative Stress - drug effects; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Rats; Free radical scavenging test; Echinacea purpurea polysaccharide; Oxidative damage model; Antioxidant activity
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.01.129

Echinacea purpurea polysaccharides (EPPS) were extracted through water extract and alcohol precipitate method. Three polysaccharides were purified by DEAE cellulose, named EPPS-1, EPPS-2 and EPPS-3. The antioxidant activities in three polysaccharides were screened by free radical scavenging test and EPPS-3 possessed the best antioxidant function. Then the antioxidant activities of EPPS-3 were further explored in oxidative damage model in vitro and in vivo for the first time. The results showed that the antioxidases and the metabolism content were significantly improved in EPPS-3 group. EPPS-3 could protect hepatic tissue from the injury of CCl4 compared with the oxidative damage model. The mechanism research demonstrated that EPPS-3 restrained cell apoptosis and promoted Nrf2 cell signal pathway to play an antioxidant impact. Therefore, EPPS-3 an ingredient could be served as amazing gift for food industry and feed additive.