Modulation of Human Subcutaneous Adipose Tissue MicroRNA Profile Associated with Changes in Adiposity‐Related Parameters

• Published in: Molecular nutrition & food research Vol. 62; no. 2
• Main Authors: Giardina, Simona, Hernández‐Alonso, Pablo, Salas‐Salvadó, Jordi, Rabassa‐Soler, Antoni, Bulló, Mònica
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-01-2018
• Subjects: Adipose tissue; Adiposity - genetics; Adiposity - physiology; Adult; Anthropometry; Body fat; Body weight; Body Weight - genetics; Caloric Restriction; Carbohydrates; Diet; Diet, Fat-Restricted; Dietary restrictions; Female; Genomes; Glycemic Index; high‐throughput; Humans; Hypocaloric diet; Male; MicroRNAs; MicroRNAs - genetics; miRNA; Nutrient deficiency; obesity; Obesity - diet therapy; Obesity - genetics; Overweight; Overweight - diet therapy; Overweight - genetics; post‐transcriptional regulation; Ribonucleic acid; RNA; Subcutaneous Fat - physiology; Transcriptome; Waist Circumference - genetics; carbohydrates; diet; post-transcriptional regulation; obesity; high-throughput
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201700594

Scope
To analyze the effect of three calorie‐restricted diets with different amount and quality of carbohydrates on subcutaneous adipose tissue (SAT) microRNA (miRNA) profile.
Methods and results
6‐month parallel, randomized trial conducted on overweight and obese subjects randomized to: 1) low glycemic index diet (LGI), 2) high glycemic index diet (HGI), and 3) low‐fat (LF). The genome‐wide SAT miRNA profile was assessed in eight randomly selected participants and the most relevant changing miRNAs (n = 13) were validated in 48 subjects. None of the miRNAs showed significant changes between the intervention groups. However, changes in some of them correlated with changes in biochemical and anthropometric variables. Stratifying our population according to tertiles of percentage change in body weight (BW), we observed a significant down‐regulation of miR‐210 in those subjects in Tertile 1 as compared to Tertile 3. When our population was stratified by tertiles of waist circumference, miR‐132, miR‐29a, miR‐34a, and miR‐378 were found to be significantly down‐regulated, in T2 compared to T3. Furthermore, when stratified by tertiles of fat mass, we also observed the significant down‐regulation of miR‐132 in T1.
Conclusion
The macronutrient composition of a calorie‐restricted diet does not affect the expression of the miRNAs analyzed, while changes in adiposity play a primary regulatory role.
Adipose tissue microRNAs expression changes in response to weight loss. However, it is not clear whether the change is due to the magnitude of the weight loss or the way in which it is lost. To better clarify this issue, the effect of three energy‐restricted diets that differ in the amount and quality of carbohydrates on subcutaneous adipose tissue miRNAs expression was analyzed.